Sadeghzadeh Zahra, Khosravi Ayyoob, Jazi Marie Saghaeian, Asadi Jahanbakhsh
Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Glycoconj J. 2020 Jun;37(3):319-327. doi: 10.1007/s10719-020-09917-z. Epub 2020 Mar 10.
Recently, studies have shown that Fucosylation plays an important role in the invasion and metastatic process of CSLCs. Understanding the expression pattern of fucosyltransferase (FUT) genes may help to suggest better-targeted therapy strategies for esophageal squamous cell carcinoma (ESCC). The study aimed to address the expression pattern of FUT gene variants in esophageal CSLCs and parental adherent cells. Sphere formation method was used to enrich CSLCs. Expression of FUT genes was examined in tumor sphere and parental adherent cells using the RT-PCR method and then relative expression of detected variants was performed by the Real-Time PCR method in both groups. The detected FUTs, also, were assessed in fresh ESCC tumors and the matched healthy controls. Analysis of The cell surface carbohydrate Lewis x (LeX, CD15) was performed by flow cytometry. Molecular analysis showed that the expression of FUT 3, 8 and POFUT1, 2 genes in tumorsphere were significantly higher than parental adherent cells. Analysis of fresh ESCC tumor tissues and the matched healthy controls showed that FUT8 and POFUT1, 2 genes in contrast to FUT 3 have higher expression in tumor tissues than controls. Flow cytometric analyses revealed that tumorsphere and their parent cells do not differ significantly in Lewis x surface marker. The present study showed that FUT 3, 8 and POFUT1, 2 genes upregulated in esophageal CSLCs in comparison to adherent cells. Understanding the expression pattern of FUT gene variants may help to suggest better-targeted therapy strategies for ESCC.
最近，研究表明岩藻糖基化在癌症干细胞（CSLCs）的侵袭和转移过程中起着重要作用。了解岩藻糖基转移酶（FUT）基因的表达模式可能有助于为食管鳞状细胞癌（ESCC）提出更具针对性的治疗策略。该研究旨在探讨FUT基因变体在食管CSLCs和亲本贴壁细胞中的表达模式。采用成球法富集CSLCs。使用RT-PCR方法检测肿瘤球和亲本贴壁细胞中FUT基因的表达，然后通过实时PCR方法对两组中检测到的变体进行相对表达分析。还在新鲜的ESCC肿瘤和匹配的健康对照中评估检测到的FUTs。通过流式细胞术分析细胞表面碳水化合物Lewis x（LeX，CD15）。分子分析表明，肿瘤球中FUT 3、8和POFUT1、2基因的表达明显高于亲本贴壁细胞。对新鲜ESCC肿瘤组织和匹配的健康对照的分析表明，与FUT 3相比，FUT8和POFUT1、2基因在肿瘤组织中的表达高于对照。流式细胞术分析显示，肿瘤球及其亲本细胞在Lewis x表面标志物上没有显著差异。本研究表明，与贴壁细胞相比，食管CSLCs中FUT 3、8和POFUT1、2基因上调。了解FUT基因变体的表达模式可能有助于为ESCC提出更具针对性的治疗策略。
