• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

岩藻糖基转移酶3和8通过CD15s和E-钙黏蛋白促进食管癌中癌干细胞样细胞的转移能力。

Fucosyltransferase 3 and 8 promote the metastatic capacity of cancer stem-like cells via CD15s and E-cadherin in esophageal cancer.

作者信息

Rostami Abookheili Aliakbar, Asadi Jahanbakhsh, Khosravi Ayyoob, Gorji Ali

机构信息

Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran.

Shefa Neuroscience Research Center, Khatam Al-anbia Hospital, Tehran, Iran.

出版信息

Iran J Basic Med Sci. 2024;27(8):985-995. doi: 10.22038/IJBMS.2024.74726.16228.

DOI:10.22038/IJBMS.2024.74726.16228
PMID:38911244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11193496/
Abstract

OBJECTIVES

Esophageal cancer stem cells (ECSCs) have been identified as the subset of cells within esophageal squamous cell carcinoma that possess tumorigenic, invasive, and metastatic properties. One important aspect of cancer metastasis is the binding of sialyl-Lewis X (CD15s) with E- or P-selectin, which facilitates the adhesion and migration of cancer cells to distant sites. This study was conducted to investigate the impact of fucosylation processes on the metastatic behavior of ECSCs.

MATERIALS AND METHODS

The esophageal cancer cell line (KYSE-30) was cultured and divided into control and 2F-peracetyl fucose (2F-PerAcFuc) treated groups. Spheres were harvested from these cultures. Cell invasion assay and qPCR were conducted to examine migration and marker expression in both groups. Cancer cell line-derived xenografts were established in nude mice to validate findings .

RESULTS

Our results initially indicated that the addition of 2F-PerAcFuc, an inhibitor of fucosylation, resulted in the down-regulation of the Fut3/CD15s pathway in both cancer stem-like cells and the xenograft model. Measurements of subcutaneous xenograft tumor volume revealed a significant decrease in tumor size among nude mice after treatment with 2F-PerAcFuc. Additionally, a reduction in Fut8/E-cadherin levels was observed in the xenograft model of nude mice. Furthermore, the administration of 2F-PerAcFuc lowered the levels of fucosylated glycoconjugates in nude mice.

CONCLUSION

Our data suggest that inhibition of fucosyltransferase 3 and 8 can reduce the metastatic capacity of cancer stem-like cells by down-regulating CD15s and E-cadherin in a mouse model of esophageal cancer.

摘要

目的

食管癌干细胞（ECSCs）已被确定为食管鳞状细胞癌中具有致瘤、侵袭和转移特性的细胞亚群。癌症转移的一个重要方面是唾液酸化路易斯X（CD15s）与E-或P-选择素的结合，这促进癌细胞向远处部位的黏附和迁移。本研究旨在探讨岩藻糖基化过程对ECSCs转移行为的影响。

材料与方法

培养食管癌细胞系（KYSE-30），并分为对照组和2F-全乙酰化岩藻糖（2F-PerAcFuc）处理组。从这些培养物中收获球体。进行细胞侵袭试验和qPCR以检测两组中的迁移和标志物表达。在裸鼠中建立癌细胞系衍生的异种移植模型以验证研究结果。

结果

我们的结果初步表明，添加岩藻糖基化抑制剂2F-PerAcFuc导致癌症干细胞样细胞和异种移植模型中Fut3/CD15s途径的下调。皮下异种移植瘤体积测量显示，用2F-PerAcFuc处理后，裸鼠的肿瘤大小显著减小。此外，在裸鼠的异种移植模型中观察到Fut8/E-钙黏蛋白水平降低。此外，给予2F-PerAcFuc降低裸鼠中岩藻糖基化糖缀合物的水平。

结论

我们的数据表明，在食管癌小鼠模型中，抑制岩藻糖基转移酶3和8可通过下调CD15s和E-钙黏蛋白来降低癌症干细胞样细胞的转移能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/74f21719d07a/IJBMS-27-985-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/60879ec64847/IJBMS-27-985-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/62eafcefb394/IJBMS-27-985-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/11b1d03208f5/IJBMS-27-985-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/5b413e43456d/IJBMS-27-985-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/a8fc76418bd9/IJBMS-27-985-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/8ab16a2975ed/IJBMS-27-985-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/917426baa13d/IJBMS-27-985-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/0e803576b1f5/IJBMS-27-985-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/bd4f940e4304/IJBMS-27-985-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/46b239a7255d/IJBMS-27-985-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/74f21719d07a/IJBMS-27-985-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/60879ec64847/IJBMS-27-985-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/62eafcefb394/IJBMS-27-985-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/11b1d03208f5/IJBMS-27-985-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/5b413e43456d/IJBMS-27-985-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/a8fc76418bd9/IJBMS-27-985-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/8ab16a2975ed/IJBMS-27-985-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/917426baa13d/IJBMS-27-985-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/0e803576b1f5/IJBMS-27-985-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/bd4f940e4304/IJBMS-27-985-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/46b239a7255d/IJBMS-27-985-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cb/11193496/74f21719d07a/IJBMS-27-985-g011.jpg

相似文献

1
Fucosyltransferase 3 and 8 promote the metastatic capacity of cancer stem-like cells via CD15s and E-cadherin in esophageal cancer.岩藻糖基转移酶3和8通过CD15s和E-钙黏蛋白促进食管癌中癌干细胞样细胞的转移能力。
Iran J Basic Med Sci. 2024;27(8):985-995. doi: 10.22038/IJBMS.2024.74726.16228.
2
Core fucosylation of E-cadherin enhances cell-cell adhesion in human colon carcinoma WiDr cells.E-钙黏蛋白的核心岩藻糖基化增强人结肠癌WiDr细胞间的细胞黏附。
Cancer Sci. 2009 May;100(5):888-95. doi: 10.1111/j.1349-7006.2009.01125.x. Epub 2009 Mar 11.
3
Restoration of alpha(1,2) fucosyltransferase activity decreases adhesive and metastatic properties of human pancreatic cancer cells.α(1,2)岩藻糖基转移酶活性的恢复降低了人胰腺癌细胞的黏附性和转移性。
Cancer Res. 2000 Mar 1;60(5):1449-56.
4
Posttranslational modification of E-cadherin by core fucosylation regulates Src activation and induces epithelial-mesenchymal transition-like process in lung cancer cells.核心岩藻糖基化对E-钙黏蛋白的翻译后修饰调控Src激活并诱导肺癌细胞发生上皮-间质转化样过程。
Glycobiology. 2016 Feb;26(2):142-54. doi: 10.1093/glycob/cwv089. Epub 2015 Oct 6.
5
Peritoneal colonization by human pancreatic cancer cells is inhibited by antisense FUT3 sequence.反义FUT3序列可抑制人胰腺癌细胞的腹膜定植。
Int J Cancer. 2000 Nov 15;88(4):558-65. doi: 10.1002/1097-0215(20001115)88:4<558::aid-ijc7>3.0.co;2-b.
6
Fucosyltransferase 4 and 7 mediates adhesion of non-small cell lung cancer cells to brain-derived endothelial cells and results in modification of the blood-brain-barrier: in vitro investigation of CD15 and CD15s in lung-to-brain metastasis.岩藻糖基转移酶 4 和 7 介导非小细胞肺癌细胞与脑源性内皮细胞的黏附，并导致血脑屏障的改变：肺向脑转移中 CD15 和 CD15s 的体外研究。
J Neurooncol. 2019 Jul;143(3):405-415. doi: 10.1007/s11060-019-03188-x. Epub 2019 May 18.
7
FUT8 promotes breast cancer cell invasiveness by remodeling TGF-β receptor core fucosylation.FUT8 通过重塑 TGF-β 受体核心岩藻糖基化促进乳腺癌细胞侵袭。
Breast Cancer Res. 2017 Oct 5;19(1):111. doi: 10.1186/s13058-017-0904-8.
8
CD15s/CD62E Interaction Mediates the Adhesion of Non-Small Cell Lung Cancer Cells on Brain Endothelial Cells: Implications for Cerebral Metastasis.CD15s/CD62E相互作用介导非小细胞肺癌细胞与脑内皮细胞的黏附：对脑转移的影响
Int J Mol Sci. 2017 Jul 10;18(7):1474. doi: 10.3390/ijms18071474.
9
[The inhibition effects of apatinib on cell proliferation, migration and apoptosis in esophageal carcinoma via Ras/Raf/MEK/ERK and JAK2/STAT3 pathways].阿帕替尼通过Ras/Raf/MEK/ERK和JAK2/STAT3信号通路对食管癌细胞增殖、迁移及凋亡的抑制作用
Zhonghua Zhong Liu Za Zhi. 2019 Apr 23;41(4):263-275. doi: 10.3760/cma.j.issn.0253-3766.2019.04.005.
10
Enhanced expression of alpha(1,3)-fucosyltransferase genes correlates with E-selectin-mediated adhesion and metastatic potential of human lung adenocarcinoma cells.α(1,3)-岩藻糖基转移酶基因的表达增强与E-选择素介导的人肺腺癌细胞黏附及转移潜能相关。
Cancer Res. 1998 Apr 1;58(7):1544-50.

引用本文的文献

1
Advances in cancer research on FUT8Molecular mechanisms and clinical applications.FUT8的癌症研究进展：分子机制与临床应用
Int J Surg. 2025 Jun 10;111(9):6290-304. doi: 10.1097/JS9.0000000000002669.

本文引用的文献

1
Targeting aberrant sialylation and fucosylation in prostate cancer cells using potent metabolic inhibitors.使用有效的代谢抑制剂靶向前列腺癌细胞中的异常唾液酸化和岩藻糖化。
Glycobiology. 2023 Dec 30;33(12):1155-1171. doi: 10.1093/glycob/cwad085.
2
Upregulation of Fucosyltransferase 3, 8 and protein O-Fucosyltransferase 1, 2 genes in esophageal cancer stem-like cells (CSLCs).食管癌干细胞样细胞（CSLCs）中岩藻糖基转移酶3、8及蛋白O-岩藻糖基转移酶1、2基因的上调。
Glycoconj J. 2020 Jun;37(3):319-327. doi: 10.1007/s10719-020-09917-z. Epub 2020 Mar 10.
3
Prognostic value of the FUT family in acute myeloid leukemia.
FUT 家族在急性髓系白血病中的预后价值。
Cancer Gene Ther. 2020 Feb;27(1-2):70-80. doi: 10.1038/s41417-019-0115-9. Epub 2019 Jun 17.
4
Fucosyltransferase 4 and 7 mediates adhesion of non-small cell lung cancer cells to brain-derived endothelial cells and results in modification of the blood-brain-barrier: in vitro investigation of CD15 and CD15s in lung-to-brain metastasis.岩藻糖基转移酶 4 和 7 介导非小细胞肺癌细胞与脑源性内皮细胞的黏附，并导致血脑屏障的改变：肺向脑转移中 CD15 和 CD15s 的体外研究。
J Neurooncol. 2019 Jul;143(3):405-415. doi: 10.1007/s11060-019-03188-x. Epub 2019 May 18.
5
Death receptor 5 is activated by fucosylation in colon cancer cells.结肠癌细胞中岩藻糖基化激活死亡受体 5。
FEBS J. 2019 Feb;286(3):555-571. doi: 10.1111/febs.14742. Epub 2019 Jan 14.
6
Aberrant glycosylation and cancer biomarker discovery: a promising and thorny journey.异常糖基化与癌症生物标志物的发现：充满希望与荆棘的征途。
Clin Chem Lab Med. 2019 Mar 26;57(4):407-416. doi: 10.1515/cclm-2018-0379.
7
Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF-β induced EMT in pancreatic cancer cells.敲低FUT3会破坏胰腺癌细胞的增殖、迁移、肿瘤发生以及TGF-β诱导的上皮-间质转化。
Oncol Lett. 2018 Jul;16(1):924-930. doi: 10.3892/ol.2018.8738. Epub 2018 May 18.
8
Distinct human α(1,3)-fucosyltransferases drive Lewis-X/sialyl Lewis-X assembly in human cells.不同的人α(1,3)-岩藻糖基转移酶在人细胞中驱动 Lewis-X/唾液酸化 Lewis-X 的组装。
J Biol Chem. 2018 May 11;293(19):7300-7314. doi: 10.1074/jbc.RA117.000775. Epub 2018 Mar 28.
9
Overexpression of α (1,6) fucosyltransferase in the development of castration-resistant prostate cancer cells.α（1,6）岩藻糖基转移酶过表达在去势抵抗性前列腺癌细胞发展中的作用。
Prostate Cancer Prostatic Dis. 2018 Apr;21(1):137-146. doi: 10.1038/s41391-017-0016-7. Epub 2018 Jan 16.
10
Fentanyl Promotes Breast Cancer Cell Stemness and Epithelial-Mesenchymal Transition by Upregulating α1, 6-Fucosylation via Wnt/β-Catenin Signaling Pathway.芬太尼通过Wnt/β-连环蛋白信号通路上调α1,6-岩藻糖基化促进乳腺癌细胞干性和上皮-间质转化。
Front Physiol. 2017 Jul 26;8:510. doi: 10.3389/fphys.2017.00510. eCollection 2017.