Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Used as a Biocontrol Agent Against Post-harvest Diseases.

is an effective biocontrol agent used to control post-harvest diseases of fruits and vegetables. I-182 was the active agent used in the first-generation yeast-based commercial product, Aspire, for post-harvest disease management. Several action modes, like competition for nutrients and space, induction of pathogenesis-related genes in host tissues, and production of extracellular lytic enzymes, have been demonstrated for the biological control activity exhibited by through which it inhibits post-harvest pathogens. In the present study, the whole genome of I-182 was sequenced using PacBio and Illumina shotgun sequencing technologies, yielding an estimated genome size of 14.73 Mb. The genome size is similar in length to that of the model yeast strain S288c. Based on the assembled genome, protein-coding sequences were identified and annotated. The predicted genes were further assigned with gene ontology terms and clustered in special functional groups. A comparative analysis of proteome with the proteomes of 11 representative yeasts revealed 2 unique and 124 expanded families of proteins in . Availability of the genome sequence will facilitate a better understanding the properties of biocontrol yeasts at the molecular level.