Nicolaus Copernicus University, Collegium Medicum, Faculty of Pharmacy, Department of Physical Chemistry, Kurpińskiego 5, Bydgoszcz, 85-950, Poland.
Photochem Photobiol Sci. 2020 Apr 1;19(4):473-484. doi: 10.1039/c9pp00478e. Epub 2020 Mar 11.
This study presents the full theoretical optical and biological characteristics of a new fluorescent probe based on the phenanthroimidazole backbone (PB5). The aldehyde group was selected as the active group to bind to the protein during conjugation. The new fluorescent probe is based on the phenanthroimidazole backbone; however, unlike previously presented works, as the chromophore part, it contains the first introduction of the 4-chloro-2H-chromen-2-one part. In order to achieve the best cognitive aspect, the study included not only the dye itself but also the concanavalin A conjugate. The linear and non-linear optical properties and biological activities described in this study clearly indicate that the presented dye is a promising material as a fluorescent probe in medical imaging.
本研究提出了一种基于菲并咪唑骨架（PB5）的新型荧光探针的全理论光学和生物学特性。醛基被选为活性基团，在结合过程中与蛋白质结合。新型荧光探针基于菲并咪唑骨架；然而，与以前的工作不同，作为生色团部分，它首次包含了 4-氯-2H-色烯-2-酮部分。为了达到最佳的认知效果，该研究不仅包括染料本身，还包括伴刀豆球蛋白 A 缀合物。本研究中描述的线性和非线性光学性质和生物活性清楚地表明，所提出的染料是一种很有前途的医学成像荧光探针材料。
