School of Basic Medical Science, Southwest Medical University, Luzhou 64600, China.
Department of Spine Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 64600, China.
ACS Appl Mater Interfaces. 2020 Apr 1;12(13):14971-14982. doi: 10.1021/acsami.0c02304. Epub 2020 Mar 20.
Poly(ether ether ketone) (PEEK) is a biocompatible polymer, but the lack of angiogenesis makes the long-term osteogenic fixation of PEEK implants challenging, which has hampered their wider application in orthopedics. Herein, we develop a multifunctional micro-/nanostructured surface presenting hydroxyapatite (HA) nanoflowers and nickel hydroxide (Ni(OH)) nanoparticles on PEEK implants (sPEEK-Ni-HA) to tackle the problem. The results show that the reasonable release of Ni from sPEEK-Ni-HA significantly facilitates the migration, tube formation, and angiogenic gene expression of human umbilical vein endothelial cells (HUVECs). In addition to angiogenesis, the sPEEK-Ni-HA displays enhanced cytocompatibility and osteogenicity in terms of cell proliferation, spreading, alkaline phosphatase activity, matrix mineralization, and osteogenesis-related gene secretion, exceeding pure and other multifunctional sPEEK samples. Importantly, evaluations employing a rabbit femoral condyle implantation model confirm that such dual decoration of Ni elements and HA nanoflowers boosts bone remodeling/osseointegration, which dramatically promotes the osteogenic fixation of implants. Therefore, this work not only sheds light on the significance of angiogenesis on the osteogenic fixation of an implant but also presents a facile strategy to empower bioinert PEEK with a well-orchestrated feature of angiogenesis and osteogenesis.
聚醚醚酮（PEEK）是一种生物相容性聚合物，但缺乏血管生成使得 PEEK 植入物的长期成骨固定具有挑战性，这阻碍了它们在骨科中的更广泛应用。在此，我们开发了一种多功能的微/纳米结构化表面，在 PEEK 植入物（sPEEK-Ni-HA）上呈现出羟基磷灰石（HA）纳米花和氢氧化镍（Ni(OH)）纳米颗粒，以解决这个问题。结果表明，sPEEK-Ni-HA 中合理释放的 Ni 显著促进了人脐静脉内皮细胞（HUVEC）的迁移、管形成和血管生成基因表达。除了血管生成，sPEEK-Ni-HA 在细胞增殖、铺展、碱性磷酸酶活性、基质矿化和骨生成相关基因分泌方面表现出增强的细胞相容性和成骨能力，超过了纯 sPEEK 和其他多功能 sPEEK 样品。重要的是，利用兔股骨髁植入模型的评估证实，Ni 元素和 HA 纳米花的这种双重装饰增强了骨重塑/骨整合，极大地促进了植入物的成骨固定。因此，这项工作不仅揭示了血管生成对植入物成骨固定的重要性，而且还提出了一种简单的策略，赋予生物惰性 PEEK 以协调的血管生成和成骨特性。
