Nephrology Division, Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts.
Department of Genetics, Harvard Medical School, Boston, Massachusetts.
Zebrafish. 2020 Apr;17(2):153-155. doi: 10.1089/zeb.2020.1862. Epub 2020 Mar 10.
Reactive oxygen species (ROS) are important regulators of intracellular signaling pathways in health and disease. It is implicated that ROS may play critical roles in pathogenesis of a number of kidney diseases including diabetic nephropathy. However, due to the lack of tools for detection of redox status, our knowledge of redox dynamics is still fragmentary. In this study, we present novel zebrafish UAS transgenic lines expressing mitochondrial and cytoplasmic targeted redox fluorescent biosensors, Grx1-roGFP2 and mitoGrx1-roGFP2. As the zebrafish is an ideal animal model for intravital imaging, these transgenic zebrafish provide useful tools to analyze renal redox dynamics .
活性氧（ROS）是健康和疾病中细胞内信号通路的重要调节剂。有研究表明，ROS 可能在包括糖尿病肾病在内的多种肾脏疾病的发病机制中发挥关键作用。然而，由于缺乏检测氧化还原状态的工具，我们对氧化还原动力学的了解仍然很零碎。在这项研究中，我们展示了新型斑马鱼 UAS 转基因系，表达线粒体和细胞质靶向的氧化还原荧光生物传感器 Grx1-roGFP2 和 mitoGrx1-roGFP2。由于斑马鱼是活体成像的理想动物模型，这些转基因斑马鱼为分析肾脏氧化还原动力学提供了有用的工具。
