Ferrer E, Martin M, Alonso R, Luque J
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
J Chromatogr. 1988 Jul 22;445(2):327-36. doi: 10.1016/s0021-9673(01)84545-3.
The partition of chick-embryo and young-chick erythrocytes in dextran-poly-(ethylene glycol) two-phase systems depends on the interfacial tension and electrical potential differences between the phases. Counter-current distribution with charged 5% dextran-poly(ethylene glycol) systems has proved to be an adequate method for the separation of primitive and definitive erythrocytes present in chick embryos when a phase settling time of 20 min is used. The computer-aided numerical resolution of experimental curves has shown the existence of subpopulations which could not have been detected by using conventional methods.
鸡胚和幼鸡红细胞在葡聚糖 - 聚（乙二醇）双相系统中的分配取决于两相之间的界面张力和电势差。当使用20分钟的相沉降时间时，用带电荷的5%葡聚糖 - 聚（乙二醇）系统进行逆流分配已被证明是分离鸡胚中原始红细胞和成熟红细胞的一种合适方法。实验曲线的计算机辅助数值解析表明存在一些用传统方法无法检测到的亚群。
