Ridgeway Biologicals Ltd. a Ceva Santé Animale Company, Units 1-3 Old Station Business Park, Compton, Berkshire, England, RG20 6NE, UK.
Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling, Scotland, FK9 4LA, UK.
Int J Syst Evol Microbiol. 2020 Mar;70(3):2034-2048. doi: 10.1099/ijsem.0.004009.
is a fastidious facultative intracellular bacterial pathogen that causes 'piscine francisellosis', a serious disease affecting both marine and fresh water farmed and wild fish worldwide. Currently two subspecies are recognized, i.e. subsp. and subsp. . In the present study, the taxonomy of was revisited using a polyphasic approach, including whole genome derived parameters such as digital DNA-DNA hybridization, whole genome average nucleotide identity (wg-ANIm), whole genome phylogenetic analysis, whole genome G+C content, metabolic fingerprinting and chemotaxonomic analyses. The results indicated that isolates belonging to subsp. represent a phenotypically and genetically homogenous taxon, clearly distinguishable from subsp. that fulfils requirements for separate species status. We propose, therefore, elevation of subsp. to the species rank as sp. nov. with the type strain remaining as Ehime-1 (DSM 21254=LMG 24544). Furthermore, we identified sufficient phenotypic and genetic differences between subsp. recovered from diseased farmed Atlantic salmon in Chile and those isolated from wild and farmed Atlantic cod in Northern Europe to warrant proposal of the Chilean as a novel subspecies, i.e. subsp. subsp. nov. with strain PQ1106 (CECT 9798=NCTC14375) as the type strain. Finally, we emend the description of by including further metabolic information and the description of atypical strains.
是一种挑剔的兼性细胞内细菌病原体，可引起“鱼类弗朗西斯菌病”，这是一种严重的疾病，影响全球海洋和淡水养殖及野生鱼类。目前已识别出两个亚种，即 subsp. 和 subsp. 。在本研究中，采用多相方法重新审视了 的分类学，包括全基因组衍生参数，如数字 DNA-DNA 杂交、全基因组平均核苷酸同一性 (wg-ANIm)、全基因组系统发育分析、全基因组 G+C 含量、代谢指纹图谱和化学生物分类分析。结果表明，属于 subsp. 的分离株代表一个表型和遗传上同质的分类群，与符合独立种状态要求的 subsp. 明显不同。因此，我们提议将 subsp. 提升为物种等级，即 sp. nov.，其模式株仍为 Ehime-1（DSM 21254=LMG 24544）。此外，我们在智利从患病养殖大西洋鲑鱼中分离出的 subsp. 和从北欧野生和养殖大西洋鳕鱼中分离出的 subsp. 之间发现了足够的表型和遗传差异，足以证明智利的亚种是一个新的亚种，即 subsp. subsp. nov.，其模式株为 PQ1106 株（CECT 9798=NCTC 14375）。最后，我们通过纳入更多代谢信息和描述非典型菌株来修订 的描述。
