National Laboratory of Solid State Microstructures, College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China.
Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing 210093, China.
ACS Appl Mater Interfaces. 2020 Apr 1;12(13):14797-14805. doi: 10.1021/acsami.9b21346. Epub 2020 Mar 20.
Nanoimprint lithography presents a new strategy for preparing uniform nanostructures with predefined sizes and shapes and has the potential for developing nanosized drug delivery systems. However, the current nanoimprint lithography is a type of an additive nanofabrication method that has limited potential due to its restricted template-dependent innate character. Herein, we have developed a novel subtractive UV-nanoimprint lithography (sUNL) for the scalable fabrication of PLGA nanostructures with variable sizes for the first time. sUNL can not only fabricate a variety of predefined nanostructures by simply utilizing different nanoimprint molds but also precisely prepare scalable nanocylinders with different length to diameter ratios. Particularly, sUNL can fabricate paclitaxel-loaded PLGA nanocylinders (PTX-PLGA NCs) with high drug-loading rate of 40% and long storage stability over a year. We demonstrate that PTX-PLGA NCs target clathrin- and caveolae-mediated cell transport pathways and display increased cellular uptake, in comparison to traditional PTX-loaded PLGA nanoparticles (PTX-PLGA NPs), leading to enhanced anticancer effects. Therefore, sUNL represents a promising nanofabrication method for efficiently developing predefined drug delivery systems.
纳米压印光刻技术为制备具有预定尺寸和形状的均匀纳米结构提供了一种新策略，并且具有开发纳米药物输送系统的潜力。然而，目前的纳米压印光刻技术是一种添加剂纳米制造方法，由于其受限于模板的固有特性，其潜力有限。在此，我们首次开发了一种新型的减法紫外纳米压印光刻（sUNL），用于可扩展地制造具有不同尺寸的 PLGA 纳米结构。sUNL 不仅可以通过简单地使用不同的纳米压印模具来制造各种预定的纳米结构，还可以精确地制备具有不同长径比的可扩展纳米圆柱。特别地，sUNL 可以制备载紫杉醇的 PLGA 纳米圆柱（PTX-PLGA NCs），载药率高达 40%，并且在一年以上的时间内具有良好的储存稳定性。我们证明，与传统的载紫杉醇的 PLGA 纳米粒子（PTX-PLGA NPs）相比，PTX-PLGA NCs 靶向网格蛋白和小窝蛋白介导的细胞运输途径，显示出更高的细胞摄取率，从而增强了抗癌效果。因此，sUNL 代表了一种很有前途的纳米制造方法，可用于高效开发预定的药物输送系统。
