Chun Yoojin, Do Anh, Grishina Galina, Grishin Alexander, Fang Gang, Rose Samantha, Spencer Chantal, Vicencio Alfin, Schadt Eric, Bunyavanich Supinda
Institute for Data Science and Genomic Technology, Department of Genetics and Genomic Sciences.
Division of Allergy and Immunology, Department of Pediatrics, and.
JCI Insight. 2020 Mar 12;5(5):133707. doi: 10.1172/jci.insight.133707.
Relatively little is known about interactions between the airway microbiome and airway host transcriptome in asthma. Since asthma affects and is affected by the entire airway, studying the upper (e.g., nasal) and lower (e.g., bronchial) airways together represents a powerful approach to understanding asthma. Here, we performed a systematic, integrative study of the nasal and bronchial microbiomes and nasal and bronchial host transcriptomes of children with severe persistent asthma and healthy controls. We found that (a) the microbiomes and host transcriptomes of asthmatic children are each distinct by site (nasal versus bronchial); (b) among asthmatic children, Moraxella and Alloiococcus are hub genera in the nasal microbiome, while there are no hubs among bronchial genera; (c) bronchial Actinomyces is negatively associated with bronchial genes for inflammation, suggesting Actinomyces may be protective; (d) compared with healthy children, asthmatic children express more nasal genes for ciliary function and harbor more nasal Streptococcus; and (e) nasal genera such as Corynebacterium are negatively associated with significantly more nasal genes for inflammation in healthy versus asthmatic children, suggesting a potentially stronger protective role for such nasal genera in healthy versus asthmatic children. Our systematic, integrative study provides a window into host-microbiome associations in asthma.
关于哮喘中气道微生物群与气道宿主转录组之间的相互作用，人们了解得相对较少。由于哮喘会影响整个气道并受其影响，同时研究上呼吸道（如鼻腔）和下呼吸道（如支气管）是理解哮喘的有力方法。在此，我们对重度持续性哮喘患儿和健康对照的鼻腔和支气管微生物群以及鼻腔和支气管宿主转录组进行了系统的综合研究。我们发现：（a）哮喘患儿的微生物群和宿主转录组在不同部位（鼻腔与支气管）各有差异；（b）在哮喘患儿中，莫拉克斯氏菌属和差异球菌属是鼻腔微生物群中的核心属，而支气管属中没有核心属；（c）支气管放线菌与支气管炎症相关基因呈负相关，提示放线菌可能具有保护作用；（d）与健康儿童相比，哮喘患儿表达更多鼻腔纤毛功能相关基因且鼻腔链球菌更多；（e）棒状杆菌属等鼻腔属在健康儿童与哮喘患儿中与更多鼻腔炎症相关基因呈负相关，提示此类鼻腔属在健康儿童与哮喘患儿中可能具有更强的保护作用。我们的系统综合研究为哮喘中宿主 - 微生物群关联提供了一个窗口。
