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微型反向重复转座元件 (MITEs),衍生插入多态性作为分子植物育种标记系统的工具。

Miniature inverted-repeat transposable elements (MITEs), derived insertional polymorphism as a tool of marker systems for molecular plant breeding.

机构信息

Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad, 580 005, India.

出版信息

Mol Biol Rep. 2020 Apr;47(4):3155-3167. doi: 10.1007/s11033-020-05365-y. Epub 2020 Mar 11.

Abstract

Plant molecular breeding is expected to give significant gains in cultivar development through development and utilization of suitable molecular marker systems for genetic diversity analysis, rapid DNA fingerprinting, identification of true hybrids, trait mapping and marker-assisted selection. Transposable elements (TEs) are the most abundant component in a genome and being used as genetic markers in the plant molecular breeding. Here, we review on the high copious transposable element belonging to class-II DNA TEs called "miniature inverted-repeat transposable elements" (MITEs). MITEs are ubiquitous, short and non-autonomous DNA transposable elements which have a tendency to insert into genes and genic regions have paved a way for the development of functional DNA marker systems in plant genomes. This review summarises the characteristics of MITEs, principles and methodologies for development of MITEs based DNA markers, bioinformatics tools and resources for plant MITE discovery and their utilization in crop improvement.

摘要

植物分子育种有望通过开发和利用合适的分子标记系统,进行遗传多样性分析、快速 DNA 指纹鉴定、鉴定真实杂种、性状定位和标记辅助选择,从而在品种培育方面取得重大进展。转座元件 (TEs) 是基因组中最丰富的组成部分,并且被用作植物分子育种中的遗传标记。在这里,我们回顾了属于第二类 DNA TEs 的高丰度转座元件,称为“微型反向重复转座元件” (MITEs)。MITEs 是普遍存在的、短的非自主 DNA 转座元件,它们倾向于插入基因和基因区域,为植物基因组中功能性 DNA 标记系统的发展铺平了道路。本综述总结了 MITEs 的特征、基于 MITEs 的 DNA 标记的开发原则和方法、植物 MITE 发现的生物信息学工具和资源,以及它们在作物改良中的利用。

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