Zhao Guanghong, Zhou Lizhi, Li Bo, Gu Changming
a School of Resources and Environmental Engineering, Anhui University , Hefei , P.R. China .
b Anhui Biodiversity Information Center , Hefei , P.R. China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1746-7. doi: 10.3109/19401736.2014.962238. Epub 2014 Sep 22.
The Grey nightjar Caprimulgus jotaka is a species of migratory nightjar found in East Asia. In this study, we determined its complete mtDNA by PCR-based method. The mitochondrial genome of Caprimulgus jotaka is a 17,536 bp circular molecule with the A + T content of 53.82%, which contains 37 typical mitochondrial genes (13 protein-coding genes, 2 rRNAs and 22 tRNAs) and a non-coding control region (D-loop). All protein-coding genes are initiated by ATG codon, except for the ND1 gene starts with GTG codon, and ND3 uses ATA codon. TAA is the most frequent stop codon. All tRNAs possess the classic cloverleaf secondary structure except for tRNA(Ser(AGY)) and tRNA(Leu(CUN)), which lacks the ''DHU'' stem. The D-loop is a 1969 bp long AT-rich region, which located between tRNA(Glu) and tRNA(Phe).
灰夜莺(Caprimulgus jotaka)是一种在东亚发现的候鸟。在本研究中,我们通过基于PCR的方法确定了其完整的线粒体DNA。灰夜莺的线粒体基因组是一个17536 bp的环状分子,A+T含量为53.82%,包含37个典型的线粒体基因(13个蛋白质编码基因、2个rRNA和22个tRNA)以及一个非编码控制区(D-loop)。所有蛋白质编码基因均由ATG密码子起始,除了ND1基因以GTG密码子起始,ND3使用ATA密码子。TAA是最常见的终止密码子。除了tRNA(Ser(AGY))和tRNA(Leu(CUN))缺乏“DHU”茎外,所有tRNA都具有经典的三叶草二级结构。D-loop是一个1969 bp长的富含AT的区域,位于tRNA(Glu)和tRNA(Phe)之间。