Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China.
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, 610031, China.
Angew Chem Int Ed Engl. 2020 Jun 22;59(26):10327-10331. doi: 10.1002/anie.201916718. Epub 2020 Apr 6.
The chromosome periphery (CP) is a complex network that covers the outer surface of chromosomes. It acts as a carrier of nucleolar components, helps maintain chromosome structure, and plays an important role in mitosis. Current methods for fluorescence imaging of CP largely rely on immunostaining. We herein report a small-molecule fluorescent probe, ID-IQ, which possesses aggregation-induced emission (AIE) property, for CP imaging. By labelling the CP, ID-IQ sharply highlighted the chromosome boundaries, which enabled rapid segmentation of touching and overlapping chromosomes, direct identification of the centromere, and clear visualization of chromosome morphology. ID-IQ staining was also compatible with fluorescence in situ hybridization and could assist the precise location of the gene in designated chromosome. Altogether, this study provides a versatile cytogenetic tool for improved chromosome analysis, which greatly benefits the clinical diagnostic testing and genomic research.
染色体外周（CP）是覆盖染色体外表面的复杂网络。它作为核仁成分的载体，有助于维持染色体结构，并在有丝分裂中发挥重要作用。目前用于 CP 荧光成像的方法在很大程度上依赖于免疫染色。本文报道了一种小分子荧光探针 ID-IQ，它具有聚集诱导发射（AIE）特性，可用于 CP 成像。通过对 CP 的标记，ID-IQ 清晰地突出了染色体边界，从而能够快速分割接触和重叠的染色体，直接识别着丝粒，并清晰地显示染色体形态。ID-IQ 染色也与荧光原位杂交兼容，并能协助指定染色体中基因的准确定位。总之，这项研究为改进的染色体分析提供了一种通用的细胞遗传学工具，这极大地有益于临床诊断检测和基因组研究。
