Qiu C, Wang Y, Sun J H, Qian W J, Xie H, Ding Y Q, Ding Z T
Tea Research Institute, Qingdao Agricultural University, Changcheng Road, Chengyang District, Qingdao, Shandong, 266109 China.
Mol Biol (Mosk). 2020 Jan-Feb;54(1):164-176. doi: 10.31857/S0026898420010127.
Lysine succinylation of proteins has potential impacts on protein structure and function, which occurs on post-translation level. However, the information about the succinylation of proteins in tea plants is limited. In the present study, the significant signal of succinylation in tea plants was found by western blot. Subsequently, we performed a qualitative analysis to globally identify the lysine succinylation of proteins using high accuracy nano LC-MS/MS combined with affinity purification. As a result, a total of 142 lysine succinylation sites were identified on 86 proteins in tea leaves. The identified succinylated proteins were involved in various biological processes and a large proportion of the succinylation sites were presented on proteins in the primary metabolism, including glyoxylate and dicarboxylate metabolism, TCA cycle and glycine, serine and threonine metabolism. Moreover, 10 new succinylation sites were detected on histones in tea leaves. The results suggest that succinylated proteins in tea plants might play critical regulatory roles in biological processes, especially in the primary metabolism. This study not only comprehensively analyzed the lysine succinylome in tea plants, but also provided valuable information for further investigating the functions of lysine succinylation in tea plants.
蛋白质的赖氨酸琥珀酰化作用会对蛋白质的结构和功能产生潜在影响，该过程发生在翻译后水平。然而，关于茶树中蛋白质琥珀酰化作用的信息却很有限。在本研究中，通过蛋白质免疫印迹法发现了茶树中显著的琥珀酰化信号。随后，我们运用高精度纳米液相色谱-串联质谱联用技术结合亲和纯化法，对蛋白质的赖氨酸琥珀酰化作用进行了定性分析，以全面鉴定其修饰情况。结果显示，在茶叶的86种蛋白质上共鉴定出142个赖氨酸琥珀酰化位点。所鉴定出的琥珀酰化蛋白质参与了多种生物学过程，并且大部分琥珀酰化位点存在于初级代谢中的蛋白质上，包括乙醛酸和二羧酸代谢、三羧酸循环以及甘氨酸、丝氨酸和苏氨酸代谢。此外，在茶叶组蛋白上还检测到10个新的琥珀酰化位点。这些结果表明，茶树中的琥珀酰化蛋白质可能在生物学过程中，尤其是在初级代谢中发挥关键的调控作用。本研究不仅全面分析了茶树中的赖氨酸琥珀酰化蛋白质组，还为进一步探究赖氨酸琥珀酰化在茶树中的功能提供了有价值的信息。
