Shen Chenjia, Xue Jie, Sun Tao, Guo Hong, Zhang Lei, Meng Yijun, Wang Huizhong
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27607.
Sci Rep. 2016 Feb 23;6:21764. doi: 10.1038/srep21764.
Protein lysine succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine succinylation in Taxus × media involved a diverse array of metabolic processes and protein-protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine succinylation, suggesting the presence of a common mechanism underlying the participation of succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of succinylation.
蛋白质赖氨酸琥珀酰化是真核细胞和原核细胞中普遍存在的一种蛋白质翻译后修饰，是各种代谢过程的重要调节因子。然而，对于其在南方红豆杉（一种紫杉醇含量高的杂交红豆杉物种）中的功能和细胞分布知之甚少。在本研究中，采用液相色谱-串联质谱法（LC-MS/MS）来鉴定用高效琥珀酰赖氨酸抗体免疫纯化富集的肽段。共鉴定出193个琥珀酰化蛋白和325个琥珀酰化位点。生物信息学分析表明，这些琥珀酰化蛋白参与了从代谢到蛋白质结合的广泛细胞功能，并表现出不同的亚细胞定位。此外，我们的研究结果表明，南方红豆杉中的赖氨酸琥珀酰化涉及多种代谢过程和蛋白质-蛋白质相互作用。许多参与多种代谢途径（如糖酵解、丙酮酸代谢、三羧酸循环和碳固定）的酶被鉴定为赖氨酸琥珀酰化的底物，这表明琥珀酰化参与代谢调节存在共同机制。这些结果首次全面展示了南方红豆杉的琥珀酰化蛋白质组，可能会推动未来对琥珀酰化的生物学研究。
