Yang Ye-Hong, Wu Song-Feng, Zhu Yun-Ping, Yang Jun-Tao, Liu Jiang-Feng
State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, China.
Proteomics. 2022 Sep;22(17):e2100381. doi: 10.1002/pmic.202100381. Epub 2022 Jun 9.
The lysine succinylation (Ksucc) is involved in many core energy metabolism pathways and affects the metabolic process in mitochondria, making this modification highly valuable for studying diseases related to mitochondrial disorders. In this paper, we used liquid chromatography with tandem mass spectrometry (LC-MS/MS) to perform the first global profiling of succinylation in human lungs under normal physiological conditions. Using an MS-based platform, we identified 1485 Ksucc sites in 568 proteins. We then compared these sites with those previously identified in human succinylome studies to investigate specific succinylated proteins and identify their possible functions in the lung and to explore the substrate preferences of succinylation modifiers in different cell lines and at different subcellular localizations. Our work expands the succinylation database and supplementary materials on the human succinylome and will thus help in further study of the function of Ksucc and regulation under related physiological and pathological conditions.
赖氨酸琥珀酰化（Ksucc）参与许多核心能量代谢途径，并影响线粒体中的代谢过程，使得这种修饰对于研究与线粒体疾病相关的疾病具有很高的价值。在本文中，我们使用液相色谱-串联质谱法（LC-MS/MS）在正常生理条件下首次对人肺中的琥珀酰化进行了全基因组分析。利用基于质谱的平台，我们在568种蛋白质中鉴定出1485个Ksucc位点。然后，我们将这些位点与先前在人类琥珀酰化组研究中鉴定出的位点进行比较，以研究特定的琥珀酰化蛋白质，确定它们在肺中的可能功能，并探索琥珀酰化修饰剂在不同细胞系和不同亚细胞定位中的底物偏好。我们的工作扩展了人类琥珀酰化组的琥珀酰化数据库和补充材料，因此将有助于进一步研究Ksucc在相关生理和病理条件下的功能及调控。
