Unidad Académica de Ciencias Biológicas, Universidad Autónoma de Zacatecas "Francisco García Salinas", Zacatecas, Zacatecas, México.
Gerencia de Desarrollo de Materiales y Productos Químicos, Instituto Mexicano del Petróleo, Ciudad de México, México.
PLoS One. 2020 Mar 12;15(3):e0230275. doi: 10.1371/journal.pone.0230275. eCollection 2020.
The ability of Uropathogenic Escherichia coli (UPEC) to form biofilms, can be considered an important factor that contributes to the prevalence of Urinary Tract Infections (UTIs) due to the inaccessibility of the antibiotics into the highly complex structure of the biofilm. Moreover, with the appearance of antibiotic multiresistant UPEC strains, the alternatives of treatment of UTIs are less. Silver nanoparticles (AgNPs) can be useful in the treatment of the UPEC infections due to its physicochemical properties that confer them antibacterial activity against both planktonic and biofilm structured cells. A diversity of biological methods for synthesis of AgNPs with antimicrobial activity has been widely investigated during the last decades, between these methods; the fungal-biosynthesis of AgNPs highlights as an ecofriendly, scalable and low cost method. In this study, biogenic AgNPs were synthesized with extracellular metabolites secreted by the soil fungal strain Fusarium scirpi (Ag0.5-5) by an ecofriendly, simple and efficient method. The antimicrobial activity of the biosynthesized AgNPs against UPEC was evaluated. The Minimal Inhibitory Concentration (MIC) of biogenic AgNPs over planktonic UPEC cells was 25 mg/mL, whereas a sub-MIC concentration (7.5 mg/L) was sufficient to inhibit the UPEC-biofilm formation about a 97%, or produce the disruption of an 80% of mature UPEC-biofilms demonstrating the potential of fungal-derived AgNPs to prevent UPEC infections.
尿路致病性大肠杆菌（UPEC）形成生物膜的能力，被认为是导致尿路感染（UTIs）流行的一个重要因素，因为抗生素无法进入生物膜这种高度复杂的结构。此外，随着具有抗生素多耐药性的 UPEC 菌株的出现，UTIs 的治疗选择变得更少。由于银纳米粒子（AgNPs）具有物理化学性质，使其对浮游和生物膜结构细胞都具有抗菌活性，因此在治疗 UPEC 感染方面可能会很有用。在过去几十年中，人们广泛研究了各种具有抗菌活性的生物合成 AgNPs 的方法，其中真菌生物合成 AgNPs 作为一种环保、可扩展且低成本的方法而备受关注。在这项研究中，通过一种环保、简单且高效的方法，利用土壤真菌菌株尖孢镰刀菌（Fusarium scirpi）分泌的胞外代谢物合成了生物源 AgNPs（Ag0.5-5）。评估了生物合成的 AgNPs 对 UPEC 的抗菌活性。生物源 AgNPs 对浮游 UPEC 细胞的最小抑菌浓度（MIC）为 25 mg/mL，而亚 MIC 浓度（7.5 mg/L）足以抑制 UPEC 生物膜的形成，抑制率约为 97%，或破坏 80%成熟的 UPEC 生物膜，这表明真菌衍生的 AgNPs 具有预防 UPEC 感染的潜力。
