Beltrán Pineda Mayra Eleonora, Lizarazo Forero Luz Marina, Sierra Cesar A
Grupo de investigación en Macromoléculas UN- Grupo de investigación Biología ambiental UPTC- Grupo de investigación Gestión ambiental Universidad de Boyacá - Tunja, Colombia.
Universidad Pedagógica y Tecnológica de Colombia- Grupo de investigación Biología ambiental, Tunja, Colombia.
Heliyon. 2023 Nov 30;10(1):e23108. doi: 10.1016/j.heliyon.2023.e23108. eCollection 2024 Jan 15.
Using biopolymers functionalized with antibacterial agents to manufacture active packaging is a clean alternative to mitigate food losses due to postharvest plant diseases. In this study, two mycosynthetized AgNPs impregnation methodologies on cotton (cationization and biochemical reduction) were used to obtain the antibacterial fibers (A-AgNPs-C and A-AgNPs-IBR), which, in addition to being characterized by SEM-EDX, XRD, were evaluated as antibacterial materials. The cotton fibers showed growth inhibition of at 48 h. The reuse tests of these cotton fibers showed that the two types of fibers could have up to three successive uses without losing their effectiveness, regardless of the impregnation method used. Is important to highlight that the retention tests indicated that the AgNPs remain attached to the A-AgNPs-C and A-AgNPs-IBR fibers after several successive washes. Finally, the mycosynthesized AgNPs were also impregnated on fique fibers (Fique-AgNPs) by cationization to obtain little antibacterial sacks. Nanostructured materials that in tests on potatoes showed only 7.8 % of affectation, while the tubers stored in the traditional sacks had an affectation of 25 %. This immobilization of AgNPs in natural fibers will allow the development of a nanobiotechnological application in the storage and transport of potatoes, after performing some additional cytotoxicity tests to guarantee its safety.
使用用抗菌剂功能化的生物聚合物制造活性包装是减轻因采后植物病害造成的食物损失的一种清洁替代方法。在本研究中，采用两种在棉花上的真菌合成银纳米颗粒浸渍方法（阳离子化和生化还原）来获得抗菌纤维（A-AgNPs-C和A-AgNPs-IBR），除了通过扫描电子显微镜-能谱仪（SEM-EDX）、X射线衍射（XRD）进行表征外，还将其作为抗菌材料进行了评估。棉纤维在48小时时显示出生长抑制。这些棉纤维的重复使用测试表明，无论使用哪种浸渍方法，这两种纤维都可以连续使用多达三次而不失去其有效性。需要强调的是，保留测试表明，经过几次连续洗涤后，银纳米颗粒仍附着在A-AgNPs-C和A-AgNPs-IBR纤维上。最后，通过阳离子化将真菌合成的银纳米颗粒也浸渍在菲克纤维（Fique-AgNPs）上，以获得少量抗菌袋。在土豆测试中，纳米结构材料的感染率仅为7.8%，而储存在传统袋子中的块茎感染率为25%。在进行一些额外的细胞毒性测试以确保其安全性之后，将银纳米颗粒固定在天然纤维中，这将有助于在土豆的储存和运输中开发纳米生物技术应用。
