Division of Medicinal Chemistry and Microbiology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Division of Medicinal Chemistry and Microbiology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Bioorg Chem. 2019 Dec;93:102803. doi: 10.1016/j.bioorg.2019.02.028. Epub 2019 Feb 22.
This contribution describes the biomineralization of silver nanoparticles by the microbial reduction of Ag (I) ions using the mycelium and the cell-free extract of Penicillium cyclopium. Different techniques, such as UV-Vis, SEM, TEM, FT-IR and GPC were used to characterize the obtained nanoparticles and understand the mechanism of their biosynthesis. The SEM and TEM images demonstrated the presence of silver nanoparticles on the mycelia surface suggesting that these particles are synthesized on the fungal cell wall. FT-IR analysis of the mycelium revealed two main types of compounds (saccharides and proteins) and these molecules might be involved in the formation of silver nanoparticles on the surface of mycelium. Ultraviolet-visible spectroscopy and TEM analysis confirmed the formation of silver nanoparticles with different shapes by the cell-free extract of P. cyclopium. Their size ranges from 12 to 25 nm and possess an average size of 16 ± 6 nm. GPC analysis of this filtrate revealed a few peaks responsible for polysaccharides and proteins presence. The only protein fraction with the mass approximately to 5000 Da indicated the formation of silver nanoparticles. Polypeptide(s) as the major molecules involved in biomineralization of silver by the cell-free extract of P. cyclopium are suggested. Enzymatic synthesis of silver nanoparticles by the mycelium and the cell-free extract of P. cyclopium is excluded.
本研究描述了青霉（Penicillium cyclopium）的菌丝体和无细胞提取物通过还原 Ag（I）离子对银纳米粒子的生物矿化作用。采用紫外可见分光光度法、SEM、TEM、FT-IR 和 GPC 等不同技术对获得的纳米粒子进行了表征，以了解其生物合成的机制。SEM 和 TEM 图像表明，在菌丝体表面存在银纳米粒子，表明这些粒子是在真菌细胞壁上合成的。菌丝体的 FT-IR 分析表明存在两种主要类型的化合物（糖和蛋白质），这些分子可能参与了菌丝体表面银纳米粒子的形成。紫外可见光谱和 TEM 分析证实了青霉无细胞提取物形成了不同形状的银纳米粒子。其尺寸范围为 12-25nm，平均尺寸为 16±6nm。该滤液的 GPC 分析显示出几个负责多糖和蛋白质存在的峰。分子量约为 5000Da 的唯一蛋白质部分表明形成了银纳米粒子。推测无细胞提取物中的多肽（s）是青霉生物矿化银的主要参与分子。排除了菌丝体和青霉无细胞提取物通过酶促合成银纳米粒子的可能性。
