School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun, Jilin, 130022, People's Republic of China.
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun, Jilin, 130012, People's Republic of China.
Anal Bioanal Chem. 2024 Nov;416(27):6137-6148. doi: 10.1007/s00216-024-05440-4. Epub 2024 Jul 24.
As a new type of artificial enzyme, a nanozyme is an ideal substitute for natural enzymes and has been successfully applied in many fields. However, in the application of biomolecular detection, most nanozymes have the disadvantages of long reaction times or high detection limits, prompting researchers to search for new efficient nanozymes. In this work, the enzyme-like activities of three polyoxometalate-based iron-organic complexes (Fe(bpp), Fe(bpp)·2CHOH, and [Fe(bpp)]H[Na(MoO)]), namely, FeMo, FeMo, and FeMoNa, were analyzed. All three polyoxometalate-based iron-organic complexes were found to be capable of catalyzing hydrogen peroxide (HO) to oxidize 3,3',5,5'-tetramethylbenzidine and o-phenylenediamine, resulting in visible color changes, further exhibiting peroxidase-like activity. Results showed that FeMoNa had more active sites due to its long chain structure, endowing more prominent peroxidase-like activity compared with FeMo and FeMo. A colorimetric sensing platform for HO and ascorbic acid detection based on FeMoNa was established. The linear response range for HO detection was 0.5-100 μM, and the detection limit was 0.143 μM. The linear response for ascorbic acid detection ranges from 0 to 750 μM with a detection limit of 1.07 μM. This study provides a new perspective for developing new nanozymes and expanding the sensing and detection application of nanozymes.
作为一种新型的人工酶，纳米酶是天然酶的理想替代品，已成功应用于许多领域。然而，在生物分子检测的应用中，大多数纳米酶存在反应时间长或检测限高的缺点，促使研究人员寻找新的高效纳米酶。在这项工作中，分析了三种基于多金属氧酸盐的铁有机配合物(Fe(bpp)、Fe(bpp)·2CHOH 和 [Fe(bpp)]H[Na(MoO)])的类酶活性，即 FeMo、FeMo 和 FeMoNa。研究发现，所有三种基于多金属氧酸盐的铁有机配合物都能够催化过氧化氢（HO）氧化 3,3',5,5'-四甲基联苯胺和邻苯二胺，导致可见的颜色变化，进一步表现出过氧化物酶样活性。结果表明，由于其长链结构，FeMoNa 具有更多的活性位点，与 FeMo 和 FeMo 相比，表现出更突出的过氧化物酶样活性。建立了基于 FeMoNa 的 HO 和抗坏血酸检测的比色传感平台。HO 检测的线性响应范围为 0.5-100 μM，检测限为 0.143 μM。抗坏血酸检测的线性响应范围为 0 至 750 μM，检测限为 1.07 μM。本研究为开发新型纳米酶和扩展纳米酶的传感和检测应用提供了新的视角。
