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主要 Latex 蛋白 MdMLP423 负调控苹果对真菌侵染的防御。

Major Latex Protein MdMLP423 Negatively Regulates Defense against Fungal Infections in Apple.

机构信息

Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China.

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Xingcheng 125100, China.

出版信息

Int J Mol Sci. 2020 Mar 10;21(5):1879. doi: 10.3390/ijms21051879.

DOI:10.3390/ijms21051879
PMID:32164313
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7084931/
Abstract

Major latex proteins (MLPs) play critical roles in plants defense and stress responses. However, the roles of from apple () have not been clearly identified. In this study, we focused on the biological role of , which had been previously characterized as a potential pathogenesis-related gene. Phylogenetic analysis and conserved domain analysis indicated that is a protein with a 'Gly-rich loop' (GXGGXG) domain belonging to the Bet v_1 subfamily. Gene expression profiles showed that is mainly expressed in flowers. In addition, the expression of was significantly inhibited by f. sp. (BB) and apple pathotype (AAAP) infections. Apple calli overexpressing had lower expression of resistance-related genes, and were more sensitive to infection with BB and AAAP compared with non-transgenic calli. RNA-seq analysis of -overexpressing calli and non-transgenic calli indicated that regulated the expression of a number of differentially expressed genes (DEGs) and transcription factors, including genes involved in phytohormone signaling pathways, cell wall reinforcement, and genes encoding the defense-related proteins, AP2-EREBP, WRKY, MYB, NAC, Zinc finger protein, and ABI3. Taken together, our results demonstrate that negatively regulates apple resistance to BB and AAAP infections by inhibiting the expression of defense- and stress-related genes and transcription factors.

摘要

主要乳胶蛋白（MLPs）在植物防御和应激反应中发挥着关键作用。然而，苹果中的 （）的作用尚未明确确定。在这项研究中，我们专注于 作为一种潜在的发病相关基因的生物学作用。系统发生分析和保守结构域分析表明， 是一种具有“富含甘氨酸环”（GXGGXG）结构域的蛋白质，属于 Bet v_1 亚家族。基因表达谱显示， 主要在花朵中表达。此外， 表达受 f. sp. （BB）和 苹果致病型（AAAP）感染的显著抑制。过表达 的苹果愈伤组织中抗性相关基因的表达水平较低，与非转基因愈伤组织相比，对 BB 和 AAAP 的感染更为敏感。对过表达愈伤组织和非转基因愈伤组织的 RNA-seq 分析表明， 调控了许多差异表达基因（DEGs）和转录因子的表达，包括参与植物激素信号通路、细胞壁强化以及防御相关蛋白、AP2-EREBP、WRKY、MYB、NAC、锌指蛋白和 ABI3 编码基因的表达。综上所述，我们的研究结果表明， 通过抑制防御和应激相关基因和转录因子的表达，负调控苹果对 BB 和 AAAP 感染的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df7/7084931/9ed6dce2fd75/ijms-21-01879-g010.jpg
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