Yu Xian, Wilson Richard, Eyles Alieta, Balotf Sadegh, Tegg Robert Stephen, Wilson Calum Rae
New Town Research Laboratories, Tasmanian Institute of Agriculture, University of Tasmania, New Town, TAS 7008, Australia.
Central Science Laboratory, University of Tasmania, Hobart, TAS 7005, Australia.
Proteomes. 2023 Feb 9;11(1):7. doi: 10.3390/proteomes11010007.
For potato crops, host resistance is currently the most effective and sustainable tool to manage diseases caused by the plasmodiophorid . Arguably, zoospore root attachment is the most critical phase of infection; however, the underlying mechanisms remain unknown. This study investigated the potential role of root-surface cell-wall polysaccharides and proteins in cultivars resistant/susceptible to zoospore attachment. We first compared the effects of enzymatic removal of root cell-wall proteins, -linked glycans and polysaccharides on attachment. Subsequent analysis of peptides released by trypsin shaving (TS) of root segments identified 262 proteins that were differentially abundant between cultivars. These were enriched in root-surface-derived peptides but also included intracellular proteins, e.g., proteins associated with glutathione metabolism and lignin biosynthesis, which were more abundant in the resistant cultivar. Comparison with whole-root proteomic analysis of the same cultivars identified 226 proteins specific to the TS dataset, of which 188 were significantly different. Among these, the pathogen-defence-related cell-wall protein stem 28 kDa glycoprotein and two major latex proteins were significantly less abundant in the resistant cultivar. A further major latex protein was reduced in the resistant cultivar in both the TS and whole-root datasets. In contrast, three glutathione -transferase proteins were more abundant in the resistant cultivar (TS-specific), while the protein glucan endo-1,3-beta-glucosidase was increased in both datasets. These results imply a particular role for major latex proteins and glucan endo-1,3-beta-glucosidase in regulating zoospore binding to potato roots and susceptibility to .
对于马铃薯作物而言，寄主抗性是目前管理由根肿菌引起的病害最有效且可持续的手段。可以说，游动孢子附着在根部是感染过程中最关键的阶段；然而，其潜在机制仍不清楚。本研究调查了根表面细胞壁多糖和蛋白质在对游动孢子附着具有抗性/易感性的品种中的潜在作用。我们首先比较了酶解去除根细胞壁蛋白质、连接聚糖和多糖对游动孢子附着的影响。随后对根段胰蛋白酶刮削（TS）释放的肽段进行分析，鉴定出262种在不同品种间丰度有差异的蛋白质。这些蛋白质在根表面来源的肽段中富集，但也包括细胞内蛋白质，例如与谷胱甘肽代谢和木质素生物合成相关的蛋白质，它们在抗性品种中更为丰富。将这些结果与相同品种的全根蛋白质组分析进行比较，确定了TS数据集中特有的226种蛋白质，其中188种有显著差异。在这些蛋白质中，与病原体防御相关的细胞壁蛋白茎28 kDa糖蛋白和两种主要的乳胶蛋白在抗性品种中的丰度显著降低。在TS和全根数据集中，抗性品种中另一种主要乳胶蛋白的含量均有所降低。相比之下，三种谷胱甘肽转移酶蛋白在抗性品种中（TS特有）更为丰富，而葡聚糖内切-1,3-β-葡萄糖苷酶在两个数据集中均有所增加。这些结果表明主要乳胶蛋白和葡聚糖内切-1,3-β-葡萄糖苷酶在调节游动孢子与马铃薯根的结合以及对根肿菌的易感性方面具有特殊作用。
