Matsuhashi Y, Yamamoto H
Pharmaceutical Research Laboratories, Meiji Seika Kaisha, Ltd.
Jpn J Antibiot. 1988 May;41(5):523-9.
We investigated enzymatic mechanisms of resistance to aminoglycoside antibiotics in methicillin-cephem-resistant Staphylococcus aureus (MRSA) by elucidation of the structures of the enzymatic reaction products. According to the MIC data, MRSA, (46 strains) can be classified into 3 groups as follows. 1. Group I (35 strains) was highly resistant to gentamicin (GM) and tobramycin (TOB), and produced 2"-aminoglycosides phosphotransferase (APH (2"]. 2. Group II (8 strains) was sensitive to GM, but was highly resistant to TOB, and produced 4'-aminoglycosides adenylyl-transferase (AAD (4']. 3. Group III (3 strains) was sensitive to GM and TOB, but was highly resistant to kanamycin, and produced 3'-aminoglycosides phosphotransferase (APH (3']-III. Arbekacin (HBK) was the most stable antibiotic to all of the inactivating-enzymes produced by MRSA, and all MRSA were sensitive to HBK.
我们通过阐明酶促反应产物的结构，研究了耐甲氧西林头孢菌素金黄色葡萄球菌（MRSA）对氨基糖苷类抗生素的耐药酶机制。根据最低抑菌浓度（MIC）数据，MRSA（46株）可分为以下3组。1. 第I组（35株）对庆大霉素（GM）和妥布霉素（TOB）高度耐药，并产生2”-氨基糖苷磷酸转移酶（APH(2”]）。2. 第II组（8株）对GM敏感，但对TOB高度耐药，并产生4’-氨基糖苷腺苷转移酶（AAD(4’]）。3. 第III组（3株）对GM和TOB敏感，但对卡那霉素高度耐药，并产生3’-氨基糖苷磷酸转移酶（APH(3’]-III）。阿贝卡星（HBK）是对MRSA产生的所有灭活酶最稳定的抗生素，所有MRSA对HBK均敏感。
