Experimental Hematology UCM-Research Group, Veterinary Faculty, Complutense University of Madrid, Avda. Puerta de Hierro s/n, 28040, Madrid, Spain.
Department of Biochemistry and Molecular Biology, Veterinary Faculty, Complutense University of Madrid, Avda. Puerta de Hierro s/n, 28040, Madrid, Spain.
Acta Vet Scand. 2020 Mar 12;62(1):13. doi: 10.1186/s13028-020-0511-z.
Mesenchymal stem cells (MSCs) have generated a great amount of interest over the past decade as a novel therapeutic treatment for a variety of diseases. Emerging studies have indicated that MSCs could enhance the repair of injured skin in canine cutaneous wounds.
A healthy 2 years old Bodeguero Andaluz dog was presented with multiple skin bite wounds. Antibiotic and anti-inflammatory therapy was administered for 8 days. On day three, 10 allogeneic adipose-derived mesenchymal stem cells (ASCs) were intradermally injected approximately equidistant to the ASCs treated wounds. Control wounds underwent conventional treatment with a topical antibacterial ointment until wound healing and closure. Wounds, skin morphology and healing progress were monitored via serial photographs and histopathology of biopsies obtained at day seven after ASC treatment. Histopathology revealed absence of inflammatory infiltrates and presence of multiple hair follicles in contrast to the non-ASCs treated control wounds indicating that ASC treatment promoted epidermal and dermal regeneration. ASCs were identified by flow cytometry and RT-PCR. The immunomodulatory role of ASCs was evidenced by coculturing peripheral blood mononuclear cells with allogeneic ASCs. Phytohemagglutinin was administered to stimulate lymphocyte proliferation. Cells were harvested and stained with an anticanine CD3-FITC antibody. The ASCs inhibited proliferation of T lymphocytes, which was quantified by reduction of carboxyfluorescein succinimidyl ester intensity using flow cytometry.
Compared with conventional treatment, wounds treated with ASCs showed a higher regenerative capacity with earlier and faster closure in this dog.
间充质干细胞（MSCs）作为一种治疗多种疾病的新疗法，在过去十年中引起了极大的关注。新的研究表明，MSCs 可以增强犬皮肤创伤的受损皮肤的修复。
一只健康的 2 岁 Bodeguero Andaluz 犬因多处皮肤咬伤就诊。给予抗生素和抗炎治疗 8 天。第 3 天，在距离接受 MSC 治疗的伤口等距的位置皮内注射 10 个同种异体脂肪间充质干细胞（ASCs）。对照伤口接受常规治疗，即用局部抗菌软膏治疗，直至伤口愈合和闭合。通过对 ASC 治疗后第 7 天获得的活检的连续照片和组织病理学监测伤口、皮肤形态和愈合进展。组织病理学显示无炎症浸润，与未接受 ASC 治疗的对照伤口相比存在多个毛囊，表明 ASC 治疗促进了表皮和真皮的再生。通过流式细胞术和 RT-PCR 鉴定 ASC。通过将外周血单核细胞与同种异体 ASC 共培养来证明 ASC 的免疫调节作用。用植物血球凝集素刺激淋巴细胞增殖。收获细胞并用抗犬 CD3-FITC 抗体染色。ASCs 抑制 T 淋巴细胞增殖，通过流式细胞术用羧基荧光素琥珀酰亚胺酯强度的减少来定量。
与常规治疗相比，在该犬中，接受 ASC 治疗的伤口表现出更高的再生能力，更早更快地闭合。
