Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran; Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
Exp Mol Pathol. 2020 Jun;114:104414. doi: 10.1016/j.yexmp.2020.104414. Epub 2020 Mar 9.
The Growth arrest specific 8 (GAS8) and its anti-sense transcript (GAS8-AS1) are located in a genomic region that is frequently mutated in breast cancer. These transcripts have established tumor suppressor effects in some human malignancies. In the current investigation, we aimed at identification of the role of GAS8 and GAS8-AS1 in breast cancer. We measured gene expression of GAS8 and GAS8-AS1 in paired tumoral and non-tumoral tissues obtained from 88 breast cancer patients by means of real time PCR. No significant differences were identified in expressions of GAS8 and GAS8-AS1 in tumoral samples compared with non-tumoral tissues (Fold changes = 1.53 and 1.71; P values = .28 and 0.14 respectively). Transcript levels of GAS8-AS1 was significantly correlated with estrogen receptor (ER) status (P = .01). Expression of GAS8 gene was associated with history of oral contraceptive use (P = .04). The similar expressions of GAS8 and GAS8-AS1 genes in tumoral and non-tumoral tissues of breast in spite of previous reports regarding their fundamental tumor suppressor roles in other tissues show that these genes are not involved in the pathogenesis of breast cancer. So, these genes have distinct roles in diverse tissues.
生长停滞特异性基因 8（GAS8）及其反义转录本（GAS8-AS1）位于乳腺癌中经常发生突变的基因组区域。这些转录本在一些人类恶性肿瘤中具有肿瘤抑制作用。在当前的研究中，我们旨在确定 GAS8 和 GAS8-AS1 在乳腺癌中的作用。我们通过实时 PCR 测量了 88 例乳腺癌患者配对的肿瘤和非肿瘤组织中 GAS8 和 GAS8-AS1 的基因表达。与非肿瘤组织相比，肿瘤样本中 GAS8 和 GAS8-AS1 的表达没有显著差异（倍数变化分别为 1.53 和 1.71；P 值分别为.28 和 0.14）。GAS8-AS1 的转录水平与雌激素受体（ER）状态显著相关（P = 0.01）。GAS8 基因的表达与口服避孕药使用史有关（P = 0.04）。尽管先前有报道称 GAS8 和 GAS8-AS1 基因在其他组织中具有基本的肿瘤抑制作用，但在乳腺癌的肿瘤和非肿瘤组织中表达相似，表明这些基因不参与乳腺癌的发病机制。因此，这些基因在不同组织中具有不同的作用。
