Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province 450000, PR China.
Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province 450000, PR China.
Gene. 2019 Aug 20;710:140-144. doi: 10.1016/j.gene.2019.05.040. Epub 2019 May 24.
LncRNA GAS8-AS1 inhibits thyroid carcinoma, while its role in colorectal cancer (CRC) is unknown. In the present study we found that plasma GAS8-AS1 was upregulated in early stage CRC patients, and downregulation of GAS8-AS1 effectively distinguished CRC patients from healthy controls. LncRNA AFAP1-AS1 was upregulated in CRC patients and was inversely correlated with GAS8-AS1 only in CRC patients but not in healthy controls. GAS8-AS1 overexpression mediated the downregulation of AFAP1-AS1 in colon cancer cells, while AFAP1-AS1 overexpression did not significantly affect GAS8-AS1 expression. Expression level of GAS8-AS1 decreased, while expression level of AFAP1-AS1 increased with the increase of primary tumor diameters. GAS8-AS1 overexpression led to inhibited, while AFAP1-AS1 overexpression led to promoted proliferation of CRC cells, and AFAP1-AS1 overexpression reduced the inhibitory effects of GAS8-AS1 overexpression on cancer cell proliferation. Therefore, GAS8-AS may inhibit CRC cell proliferation by downregulating AFAP1-AS1.
LncRNA GAS8-AS1 抑制甲状腺癌，但其在结直肠癌（CRC）中的作用尚不清楚。在本研究中，我们发现早期 CRC 患者的血浆 GAS8-AS1 上调，下调 GAS8-AS1 可有效区分 CRC 患者与健康对照者。CRC 患者中 AFAP1-AS1 上调，仅在 CRC 患者中与 GAS8-AS1 呈负相关，而在健康对照者中则不相关。GAS8-AS1 过表达介导了结肠癌细胞中 AFAP1-AS1 的下调，而 AFAP1-AS1 过表达对 GAS8-AS1 的表达没有显著影响。GAS8-AS1 的表达水平随着原发性肿瘤直径的增加而降低，而 AFAP1-AS1 的表达水平则增加。GAS8-AS1 过表达导致 CRC 细胞增殖受到抑制，而 AFAP1-AS1 过表达则促进 CRC 细胞增殖，并且 AFAP1-AS1 过表达降低了 GAS8-AS1 过表达对癌细胞增殖的抑制作用。因此，GAS8-AS1 可能通过下调 AFAP1-AS1 抑制 CRC 细胞增殖。
