Nanfang College of Sun Yat-sen University, Guangzhou, 510970, China.
College of Life Science, Xinyang Normal University, Xinyang, 464000, China.
Sci Rep. 2020 Mar 12;10(1):4602. doi: 10.1038/s41598-020-61317-3.
In this study, two chlorophyll A/B binding protein (CAB) genes (CsCP1 and CsCP2) in tea plant were cloned. The proteins encoded by these genes belong to the external or internal antenna proteins of PS II, respectively. They may be the targets of physiological regulation for tea leaf cell PS II because they all contain multiple functional domains and modifiable sites. The CAB gene family in the tea genome consists of 25 homologous genes. We measured the expression patterns of ten genes in the CsCP1 and CsCP2 subfamily under six different stresses. CsCP1 expression was inhibited in response to 6 kinds of stress; CsCP2 expression was slightly upregulated only after cold stress and ABA treatment. However, the expression levels of CSA016997 and CSA030476 were upregulated significantly in the six stresses. The results suggested that the 10 CAB genes may have different functions in tea leaves. Moreover, changes in the expression of the 10 genes under stress appear to be related to ABA- and MeJA-dependent signalling pathways, and their responses to MeJA treatment is faster than those to ABA. In addition, we introduced our experiences for cloning the genes in the context of complex genomes.
本研究克隆了茶树中的两个叶绿素 A/B 结合蛋白（CAB）基因（CsCP1 和 CsCP2）。这两个基因编码的蛋白分别属于 PS II 的外部或内部天线蛋白，它们可能是茶树叶片 PS II 生理调节的靶标，因为它们都含有多个功能域和可修饰位点。茶树基因组中的 CAB 基因家族由 25 个同源基因组成。我们在 6 种不同胁迫条件下测定了 CsCP1 和 CsCP2 亚家族中 10 个基因的表达模式。6 种胁迫均抑制了 CsCP1 的表达，而 CsCP2 的表达仅在冷胁迫和 ABA 处理后略有上调。然而，CSA016997 和 CSA030476 的表达水平在 6 种胁迫下均显著上调。这些结果表明，这 10 个 CAB 基因在茶树叶片中可能具有不同的功能。此外，10 个基因在胁迫下的表达变化似乎与 ABA 和 MeJA 依赖的信号通路有关，它们对 MeJA 处理的反应比 ABA 更快。此外，我们还介绍了在复杂基因组中克隆基因的经验。
