Gene Positively Regulate Salt Tolerance in Transgenic × .

Salt is an important environmental stress factor, which seriously affects the growth, development and distribution of plants. Chlorophyllase plays an important role in stress response. Nevertheless, little is known about the physiological and molecular mechanism of chlorophyll (Chlase, ) genes in plants. We cloned from and found that was differentially expressed in different tissues, especially in the leaves of . To further study the role of in salt tolerance, overexpression and RNA interference transgenic lines were established in × and used for salt stress treatment and physiologic indexes studies. Overexpressing lines significantly improved tolerance to salt treatment and reduced reactive oxygen species production. RNA interference lines showed the opposite. Transcriptome analysis was performed on leaves of control and transgenic lines under normal growth conditions and salt stress to predict genes regulated during salt stress. This provides a basis for elucidating the molecular regulation mechanism of in response to salt stress and improving the tolerance of poplar under salt stress.