Huang Wenping, Wen Zhiqi, Wang Qi, Chen Renhao, Li Zhifeng, Feng Yulin, Yang Shilin
The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, China.
State Key Laboratory of Innovative Drug and Efficient Energy-saving Pharmaceutical Equipment, Nanchang, Jiangxi, China.
Biomed Chromatogr. 2020 Jul;34(7):e4828. doi: 10.1002/bmc.4828. Epub 2020 Mar 31.
Kalimeris indica (L) Sch-Bip is a medicinal plant used by the Miao ethnic group in the Guizhou province of China. It is widely used as a fresh vegetable to treat colds, diarrhea and gastric ulcers. However, few studies have been conducted on the mechanism of its effect on colds, and its quality control. The anticomplement and antitussive activities of different polar extracts of K. indica were evaluated. Fifty-nine compounds, mainly including phenols and flavonoids, were identified in K. indica extract by ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry. A method was established through ultra-high-performance liquid chromatography with a photodiode array to simultaneously determine the anticomplement and antitussive activity of five compounds in K. indica combining chemical identification with chemometrics for discrimination and quality assessment. Also, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid exhibited significantly higher anticomplementary activity than the other three compounds. The quantitative data were further analyzed by principal component analysis and orthogonal partial least-squares discriminant analysis. Heatmap visualization was conducted to clarify the distribution of the major compounds in different geographical origins. Screening pharmacological activities by a combination of chemometrics and chemical identification might be an effective method for the quality control of K. indica.
马兰（Kalimeris indica (L) Sch-Bip）是中国贵州省苗族使用的一种药用植物。它被广泛用作新鲜蔬菜来治疗感冒、腹泻和胃溃疡。然而，关于其对感冒的作用机制及其质量控制的研究很少。对马兰不同极性提取物的抗补体和镇咳活性进行了评价。通过超高效液相色谱四极杆飞行时间串联质谱法在马兰提取物中鉴定出59种化合物，主要包括酚类和黄酮类。建立了一种通过超高效液相色谱结合光电二极管阵列同时测定马兰中5种化合物抗补体和镇咳活性的方法，将化学鉴定与化学计量学相结合用于鉴别和质量评估。此外，3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸的抗补体活性明显高于其他三种化合物。通过主成分分析和正交偏最小二乘判别分析对定量数据进行了进一步分析。进行热图可视化以阐明不同地理来源中主要化合物的分布。结合化学计量学和化学鉴定筛选药理活性可能是马兰质量控制的有效方法。
