Wang Mo-Fei, Li Hao, Cui Jian, Chen Yu-Han, Cui Yong
The Department of General Surgery, The Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia Autonomous Region, China.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
Front Pharmacol. 2023 Jan 9;13:1119091. doi: 10.3389/fphar.2022.1119091. eCollection 2022.
(L.) Sch Bip () is a plant of the genus in Asteraceae, and its whole herb can be used as medicine for the treatment of intestinal inflammatory diseases. But the mechanism is not clear. Therefore, this study was designed to explore the mechanism of (KI) in colitis-associated colorectal cancer. The expression levels of miR-31-5p and proinflammatory factors were detected using THP-1 and Caco2 cells . KI could rescue the upregulation of miR-31-5p induced by IL-6 and TNF-α in Caco2 and THP-1 cells. In LPS-stimulated PMA-differentiated THP-1 cells, KI restored miR-31-5p expression by downregulating the expression of IL-6 and TNF-α. C57BL/6 mice were used to construct CAC model through the induction of azoxymethane/dextran sulfate sodium. The successfully established CAC mice were treated with water extract of KI through intragastric administration for 5 weeks. The result showed that KI could significantly reduce the atypical hyperplasia in colon tissue, and inhibit the expression of proinflammatory factors such as IL-6, TNF, IL-11, IL-7, etc. At the same time, KI could restore the level of miR-31-5p in mice, and therefore the downstream LATS2 to inhibit the development of CAC. These above results indicate that KI is a potentially effective herb medicine to prevent CAC.
(L.) Sch Bip ()是菊科属的一种植物，其全草可入药治疗肠道炎症性疾病。但其作用机制尚不清楚。因此，本研究旨在探讨(L.) Sch Bip ()在结肠炎相关结直肠癌中的作用机制。使用THP-1和Caco2细胞检测miR-31-5p和促炎因子的表达水平。(L.) Sch Bip ()可以挽救IL-6和TNF-α在Caco2和THP-1细胞中诱导的miR-31-5p上调。在LPS刺激的PMA分化的THP-1细胞中，(L.) Sch Bip ()通过下调IL-6和TNF-α的表达来恢复miR-31-5p的表达。通过给予氧化偶氮甲烷/葡聚糖硫酸钠诱导C57BL/6小鼠构建结肠炎相关结直肠癌（CAC）模型。将成功建立的CAC小鼠通过灌胃给予(L.) Sch Bip ()水提取物治疗5周。结果表明，(L.) Sch Bip ()可以显著降低结肠组织中的非典型增生，并抑制IL-6、TNF、IL-11、IL-7等促炎因子的表达。同时，(L.) Sch Bip ()可以恢复小鼠体内miR-31-5p的水平，从而抑制下游的LATS2以抑制CAC的发展。上述结果表明，(L.) Sch Bip ()是一种预防CAC的潜在有效草药。
