el Abbouyi A, Roch-Arveiller M, Marchiani C, Dahan R, Congy F, Paul J L, Giroud J P, Raichvarg D
Laboratoire de Pharmacologie et de Biochimie A, Hôpital Cochin, France.
Mech Ageing Dev. 1988 Sep;44(3):215-29. doi: 10.1016/0047-6374(88)90023-1.
The activity of RU 41740, a glycoprotein extract from Klebsiella pneumoniae has been investigated on some polymorphonuclear (PMN) functions. Chemotaxis, random migration and oxidative metabolism (assessed by chemiluminescence, O2 consumption and O2- generation) were studied in parallel. PMN were collected from adult and aged human volunteers. Experiments were performed either in vitro or in vivo in a double blind placebo assay. In both PMN populations RU 41740 enhanced oxidative metabolism either in in vivo or in vitro experiments. However, a higher and dose-related activity was observed on PMN collected from the younger subjects whereas maximal effective concentration was reached earlier with PMN collected from aged subjects. RU 41740 did not modify random migration but inhibited chemotaxis of PMN collected from the younger population in a dose-related manner. These data corroborated previous results observed on PMN collected from various animal species and suggested an interaction of RU 41740 on PMN membrane. Moreover drug-induced macrophage and lymphocyte stimulation might also explain, at least in part, the in vivo effects described in this study. Thus RU 41740 could partly account for the protective effects exerted against bacterial and fungal infections through its activity on PMN functions.
已对肺炎克雷伯菌糖蛋白提取物RU 41740对一些多形核白细胞（PMN）功能的活性进行了研究。同时研究了趋化性、随机迁移和氧化代谢（通过化学发光、氧气消耗和超氧阴离子生成进行评估）。从成年和老年人类志愿者中收集PMN。实验在体外或体内进行双盲安慰剂试验。在两个PMN群体中，无论是体内还是体外实验，RU 41740均增强了氧化代谢。然而，在从年轻受试者收集的PMN上观察到更高的且与剂量相关的活性，而从老年受试者收集的PMN则更早达到最大有效浓度。RU 41740未改变随机迁移，但以剂量相关的方式抑制了从年轻群体收集的PMN的趋化性。这些数据证实了先前在从各种动物物种收集的PMN上观察到的结果，并表明RU 41740与PMN膜存在相互作用。此外，药物诱导的巨噬细胞和淋巴细胞刺激也可能至少部分解释了本研究中描述的体内效应。因此，RU 41740可能通过其对PMN功能的活性部分解释其对细菌和真菌感染所发挥的保护作用。
