Roch-Arveiller M, el Abbouyi A, Paul J L, Smets P, Raichvarg D, Giroud J P
Int J Immunopharmacol. 1987;9(4):417-24. doi: 10.1016/0192-0561(87)90015-4.
The activity of RU 41740, a glycoprotein extract from Klebsiella pneumoniae, endowed with immuno-modulating properties, has been investigated on polymorphonuclear (PMN) leukocyte functions. This report deals with the effect of RU 41740 on oxidative metabolism (assessed by chemiluminescence, 02 consumption and O2- production) and on chemotaxis and random migration (using agarose and Boyden chamber techniques). PMNs were collected from the rat pleural cavity after induction of one acute inflammatory reaction (pleurisy induced by injection of a suspension of calcium pyrophosphate crystals). Experiments were performed in parallel after in vivo treatment or incubation in vitro. RU 41740 enhanced PMN oxidative metabolism and inhibited PMN chemotaxis while random migration was only affected using agarose assay at high concentration. This effect on PMN migration was observed with at least two attractants. These observations have been obtained either after incubation in vitro or administration in vivo. The minimal effective dose was 1 mg/kg in vivo and 0.1 microgram/ml in vitro. These data suggest that RU 41740 acts directly on PMN membrane receptors.
肺炎克雷伯菌的一种具有免疫调节特性的糖蛋白提取物RU 41740，其活性已在多形核（PMN）白细胞功能方面进行了研究。本报告探讨了RU 41740对氧化代谢（通过化学发光、氧气消耗和超氧阴离子生成进行评估）以及趋化性和随机迁移（使用琼脂糖和博伊登小室技术）的影响。在诱导一次急性炎症反应（通过注射焦磷酸钙晶体悬液诱导胸膜炎）后，从大鼠胸腔收集PMN。在体内治疗或体外孵育后平行进行实验。RU 41740增强了PMN氧化代谢并抑制了PMN趋化性，而随机迁移仅在高浓度琼脂糖试验中受到影响。至少使用两种趋化剂观察到了对PMN迁移的这种影响。这些观察结果在体外孵育后或体内给药后均已获得。体内最小有效剂量为1 mg/kg，体外为0.1微克/毫升。这些数据表明RU 41740直接作用于PMN膜受体。
