Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.
Institution of Plant Protection, Academy of Ningxia Agricultural and Forestry Science, Yinchuan, China.
Insect Sci. 2021 Apr;28(2):347-354. doi: 10.1111/1744-7917.12776. Epub 2020 Jun 15.
Even though Paranosema locustae is widely used in China as a biological agent for controlling grasshoppers, the mortality rate is initially quite low. This study sought to determine whether the simultaneous use of P. locustae and Beauveria bassiana would be a more effective control strategy. Additionally, changes in the intestinal microbial communities of migratory locusts infected with the two pathogens were analyzed to investigate the roles of gut microbes in pathogen-host interactions. The mortality rate of locusts inoculated with B. bassiana and P. locustae simultaneously was not significantly higher than expected, but the mortality rates of locusts inoculated with B. bassiana 3, 6, and 9 days after inoculation with P. locustae were significantly higher than if their effects were additive, indicating synergism. A MiSeq analysis found that Weissella was the most common bacterium, representing 41.48% and 51.62% of the total bacteria in the mid- and hindguts, respectively, and the bacterial declines were greatest during dual infections with B. bassiana and P. locustae. The appropriately timed combined application of P. locustae and B. bassiana was more effective against locusts than either treatment alone. Moreover, the combined inoculation of the two pathogens changed the gut microflora of locusts, indicating the potential relevancy of their synergistic effects on locust control.
尽管颗粒体病毒在中国被广泛用作防治蝗虫的生物制剂，但死亡率最初相当低。本研究旨在确定同时使用颗粒体病毒和球孢白僵菌是否会是一种更有效的控制策略。此外，还分析了感染两种病原体的东亚飞蝗肠道微生物群落的变化，以研究肠道微生物在病原体-宿主相互作用中的作用。同时接种球孢白僵菌和颗粒体病毒的蝗虫死亡率并没有显著高于预期，但在接种颗粒体病毒 3、6 和 9 天后接种球孢白僵菌的蝗虫死亡率明显高于相加效应，表明存在协同作用。MiSeq 分析发现，魏斯氏菌是最常见的细菌，分别占中肠和后肠总细菌的 41.48%和 51.62%，而在球孢白僵菌和颗粒体病毒双重感染时，细菌下降幅度最大。颗粒体病毒和球孢白僵菌的适时联合应用对蝗虫的防治效果优于单独使用任何一种处理方法。此外，两种病原体的联合接种改变了蝗虫的肠道微生物群，表明它们对蝗虫控制的协同作用具有潜在的相关性。
