小鼠红细胞大小与红细胞年龄相关吗？

Does red blood cell size correlate with red blood cell age in mouse?

作者信息

Vaysse J, Vassy R, Eclache V, Bladier D, Gattegno L, Pilardeau P

机构信息

Laboratoire de Biochimie, UFR de Médecine et Biologie Expérimentale, Université Paris XIII, Bobigny, France.

出版信息

Mech Ageing Dev. 1988 Sep;44(3):265-76. doi: 10.1016/0047-6374(88)90027-9.

DOI:10.1016/0047-6374(88)90027-9

PMID:3216723

Abstract

Mouse red blood cells (RBC) can be fractionated according to their size by counterflow centrifugation. The mean corpuscular hemoglobin content and the enzyme activities (ASAT, LDH, PK and acetylcholinesterase) increase when the mean corpuscular volume (MCV) rises. However, the in-vivo survival of size-separated RBC is similar whatever their MCV is; thus, counterflow centrifugation is not a suitable procedure to achieve an age fractionation of mouse RBC. Moreover, RBC subpopulations collected by counterflow centrifugation are different from those obtained when RBC are fractionated according to their density.

摘要

小鼠红细胞（RBC）可通过逆流离心法按其大小进行分级分离。当平均红细胞体积（MCV）升高时，平均红细胞血红蛋白含量和酶活性（谷草转氨酶、乳酸脱氢酶、丙酮酸激酶和乙酰胆碱酯酶）会增加。然而，无论其MCV如何，经大小分离的红细胞的体内存活率相似；因此，逆流离心法并非实现小鼠红细胞年龄分级的合适方法。此外，通过逆流离心收集的红细胞亚群与根据密度对红细胞进行分级分离时获得的亚群不同。