California NanoSystems Institute, David Geffen School of Medicine, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, USA.
Biophys J. 2011 Sep 7;101(5):1025-31. doi: 10.1016/j.bpj.2011.07.022.
A central question in cancer therapy is how individual cells within a population of tumor cells respond to drugs designed to arrest their growth. However, the absolute growth of cells, their change in physical mass, whether cancerous or physiologic, is difficult to measure directly with traditional techniques. Here, we develop live cell interferometry for rapid, real-time quantification of cell mass in cells exposed to a changing environment. We used tunicamycin induction of the unfolded protein stress response in multiple myeloma cells to generate a mass response that was temporally profiled for hundreds of cells simultaneously. Within 2 h, the treated cells were growth suppressed compared to controls, with a few cells in both populations showing a robust increase (+15%) or little change (<5%) in mass accumulation. Overall, live cell interferometry provides a conceptual advance for assessing cell populations to identify, monitor, and measure single cell responses, such as to therapeutic drugs.
癌症治疗中的一个核心问题是，在设计用于阻止肿瘤细胞生长的药物作用下，肿瘤细胞群体中的单个细胞如何做出反应。然而，细胞的绝对生长，即其物理质量的变化，无论是癌变还是生理性的，都很难用传统技术直接测量。在这里，我们开发了活细胞干涉测量法，用于快速实时定量测量暴露于不断变化环境中的细胞的细胞质量。我们使用衣霉素诱导多发性骨髓瘤细胞中的未折叠蛋白应激反应，以产生数百个细胞同时进行的时间剖析的质量反应。在 2 小时内，与对照组相比，处理过的细胞受到了生长抑制，在两个群体中都有少数细胞表现出明显的增加（+15%）或很少的变化（<5%）。总的来说，活细胞干涉测量法为评估细胞群体提供了一个概念上的进展，可以识别、监测和测量单细胞反应，例如对治疗药物的反应。
