State Key Laboratory of Bioreactor Engineering, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Key Laboratory for Ultrafine Materials of Ministry of Education, Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 200237, China.
Angew Chem Int Ed Engl. 2020 Jun 8;59(24):9586-9593. doi: 10.1002/anie.202000416. Epub 2020 Apr 2.
The functions of implants like medical devices are often compromised by the host's foreign-body response (FBR). Herein, we report the development of low-FBR materials inspired by serine-rich sericin from silk. Poly-β-homoserine (β-HS) materials consist of the hydrophilic unnatural amino acid β-homoserine. Self-assembled monolayers (SAMs) of β-HS resist adsorption by diverse proteins, as well as adhesion by cells, platelets, and diverse microbes. Experiments lasting up to 3 months revealed that, while implantation with control PEG hydrogels induced obvious inflammatory responses, collagen encapsulation, and macrophage accumulation, these responses were minimal with β-HS hydrogels. Strikingly, the β-HS hydrogels induce angiogenesis in implant-adjacent tissues. Molecular dynamics simulations indicated that the low FBR performance of β-HS results from what we term "dual hydrogen bonding hydration", wherein both the backbone amide groups and the sidechain hydroxyl groups of β-HS undergo hydration.
植入物（如医疗器械）的功能常常会受到宿主异物反应（FBR）的影响。在这里，我们报告了受丝胶中富含丝氨酸的启发而开发的低 FBR 材料。聚-β-高丝氨酸（β-HS）材料由亲水性非天然氨基酸β-高丝氨酸组成。β-HS 的自组装单层（SAM）能抵抗各种蛋白质的吸附以及细胞、血小板和各种微生物的黏附。长达 3 个月的实验表明，虽然用对照聚乙二醇水凝胶植入会引起明显的炎症反应、胶原蛋白包封和巨噬细胞积累，但用 β-HS 水凝胶则很少发生这些反应。引人注目的是，β-HS 水凝胶会在植入物相邻组织中诱导血管生成。分子动力学模拟表明，β-HS 的低 FBR 性能源于我们所谓的“双重氢键水合”，其中β-HS 的主链酰胺基团和侧链羟基都发生水合。
