Bioactive Molecules Research Laboratory, Faculty of Sciences, Lebanese University, Lebanon; Laboratoire d'Automatique et de Génie des Procédés (LAGEP), Université Claude Bernard, Lyon 1, France.
Bioactive Molecules Research Laboratory, Faculty of Sciences, Lebanese University, Lebanon.
Int J Pharm. 2020 Apr 30;580:119198. doi: 10.1016/j.ijpharm.2020.119198. Epub 2020 Mar 10.
Liposomes are lipid vesicles made of one or multiple lipid bilayers surrounding an internal aqueous core. They are broadly employed as models to study membrane structure and properties. Among these properties, liposome membrane permeability is crucial and widely assessed by fluorescence techniques. The first part of this review is devoted to describe the various techniques used for membrane permeability assessment. Attention is paid to fluorescence techniques based on vesicle leakage of self-quenching probes, dye/quencher pair or cation/ligand pair. Secondly, the membrane-active agents inducing membrane permeabilization is presented and details on their mechanisms of action are given. Emphasis is also laid on the intrinsic and extrinsic factors that can modulate the membrane permeability. Hence, a suitable liposomal membrane should be formulated according to the aim of the study and its application.
脂质体是由一个或多个脂质双层围绕内部水核组成的脂质囊泡。它们被广泛用作研究膜结构和性质的模型。在这些性质中，脂质体膜通透性至关重要，并广泛通过荧光技术进行评估。本综述的第一部分致力于描述用于评估膜通透性的各种技术。关注基于自猝灭探针、染料/猝灭剂对或阳离子/配体对的囊泡泄漏的荧光技术。其次，介绍了诱导膜通透性的膜活性试剂，并详细介绍了其作用机制。还强调了可以调节膜通透性的内在和外在因素。因此，应根据研究目的和应用来配制合适的脂质体膜。
