Abdelnaby Elshymaa Ahmed
Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza square, 12211, Egypt.
Reprod Biol. 2020 Jun;20(2):202-209. doi: 10.1016/j.repbio.2020.03.001. Epub 2020 Mar 11.
The present detailed study aimed to establish for the first time the ovarian and uterine hemodynamic change in dairy cows after the end of the puerperal period to investigate a possible association between Doppler indices and volume of blood flow. Twenty cows weighing 500-600 kg (mean 400 ± 50 kg) and 4-5 years of age (mean 3.2 ± 0.5 years) categorized into two main groups (true anestrum, with corpus luteum, n = 10) and (normal cyclic, n = 10), The period of examination started from day 39 till day 70 after calving with day after day routinely examination by b-mode and Doppler performed on both ovaries with ovarian arteries (OA) and uterus with uterine arteries (MUA) in the ipsilateral (ipsi) and contralateral (contra) side to ovulation, in addition to a thickness in horns and body was measured. Estradiol and progesterone were also measured. Results showed that both Doppler indices in the OA and MUA ipsi and contra had a positive (P ≤ 0.001) correlation with contra Doppler indices, but revealed a negative (P ≤ 0.05) correlation with ipsi and contra Doppler velocities, blood flow rate and volume in anestrum cows. Both ovarian and uterine ipsi indices showed an increase (P ≤ 0.05) in the anestrum cows, and both PSV and EDV of both arteries ipsilateral showed (P ≤ 0.05) a decrease in the anestrum cows, the ipsi and contra ovarian and uterine colored % were lower in anestrum group than the normal group. Estradiol (P ≤ 0.05) decreased in anestrum cows than the normal, while progesterone increased in the anestrum group. Conclusion, although uterine and ovarian morphology were changed in anestrum cows, the vascular system of the ovary as well as uterus underwent much more marked vascular changes, the most significant being that of blood flow velocities and volume.
本详细研究旨在首次确定奶牛产后期末卵巢和子宫的血流动力学变化，以研究多普勒指数与血流量之间的可能关联。将20头体重500 - 600千克（平均400±50千克）、年龄4 - 5岁（平均3.2±0.5岁）的奶牛分为两个主要组（真性乏情，有黄体，n = 10）和（正常发情周期，n = 10）。检查期从产犊后第39天开始至第70天，每天对双侧卵巢及其卵巢动脉（OA）和子宫及其子宫动脉（MUA）进行常规B超和多普勒检查，检查排卵同侧（ipsi）和对侧（contra），此外还测量子宫角和子宫体的厚度。同时测定雌二醇和孕酮。结果显示，OA和MUA同侧和对侧的多普勒指数与对侧多普勒指数呈正相关（P≤0.001），但与乏情期奶牛同侧和对侧的多普勒速度、血流速率和血流量呈负相关（P≤0.05）。乏情期奶牛的卵巢和子宫同侧指数均升高（P≤0.05），同侧两条动脉的PSV和EDV均降低（P≤0.05），乏情期组同侧和对侧卵巢及子宫的彩色血流百分比低于正常组。与正常奶牛相比，乏情期奶牛的雌二醇降低（P≤0.05），而孕酮在乏情期组升高。结论：尽管乏情期奶牛的子宫和卵巢形态发生了变化，但卵巢和子宫的血管系统发生了更为显著的血管变化，最显著的是血流速度和血流量的变化。
