Johnson G, Garvey J S
J Immunol Methods. 1977;15(1):29-37. doi: 10.1016/0022-1759(77)90014-x.
Defined conditions for simplified Affinity Chromatography suitable for instructional and research purposes, were developed. The standard method of activation of polysaccharide gels using BrCN was modified and improved using a highly buffered reaction solution. A batchwise protocol for the immunoadsorbent isolation of anti-BSA was used (with commonly employed dissociation agents) and comparative data were obtained.
开发了适用于教学和研究目的的简化亲和色谱的特定条件。使用高度缓冲的反应溶液对使用溴化氰激活多糖凝胶的标准方法进行了改进。采用分批方案进行抗牛血清白蛋白的免疫吸附剂分离（使用常用的解离剂）并获得了比较数据。
