针对流产布鲁氏菌脂多糖的牛单克隆抗体。
Bovine monoclonal antibody specific for Brucella abortus lipopolysaccharide.
作者信息
Nielsen K H, Henning M D
机构信息
Agriculture Canada, Animal Diseases Research Institute Nepean, Ont.
出版信息
Vet Immunol Immunopathol. 1989 Jul;21(3-4):363-72. doi: 10.1016/0165-2427(89)90043-3.
The development of a bovine monoclonal antibody against Brucella abortus smooth lipopolysaccharide (BM-8) by interspecies fusion of bovine peripheral lymphocytes from an immunized cow and a murine plasmacytoma cell line is described. The twice cloned cell line secreted bovine IgG1 subclass antibody. Ascites fluid was prepared in pristane treated nu/nu mice by intraperitoneal injection. The pooled ascites fluid was purified by affinity chromatography and the functions of the antibody assessed in various serological tests. The BM-8 antibody did not agglutinate well at a neutral pH, however, under acid conditions it was efficient at agglutinating B. abortus cells. The antibody did not precipitate B. abortus LPS in double agar gel immunodiffusion but was very active in the direct complement fixation test and the indirect enzyme immunoassay, although it was unable to compete with a murine monoclonal antibody in a competitive enzyme immunoassay.
本文描述了通过将免疫牛的外周淋巴细胞与鼠骨髓瘤细胞系进行种间融合来开发一种抗流产布鲁氏菌光滑脂多糖的牛单克隆抗体（BM-8）的过程。经过两次克隆的细胞系分泌牛IgG1亚类抗体。通过腹腔注射在经 pristane 处理的裸鼠中制备腹水。合并的腹水通过亲和层析进行纯化，并在各种血清学试验中评估抗体的功能。BM-8抗体在中性pH下凝集效果不佳，但在酸性条件下能有效凝集流产布鲁氏菌细胞。该抗体在双琼脂凝胶免疫扩散中不沉淀流产布鲁氏菌脂多糖，但在直接补体结合试验和间接酶免疫测定中非常活跃，尽管在竞争酶免疫测定中它无法与鼠单克隆抗体竞争。
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