牛抗布鲁氏菌流产亚种脂多糖抗体的亲和纯化。
Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide.
作者信息
Stiller J M, Nielsen K H
出版信息
J Clin Microbiol. 1983 Feb;17(2):323-6. doi: 10.1128/jcm.17.2.323-326.1983.
Abstract
Alkali-treated lipopolysaccharide from Brucella abortus S1119.3 coupled to agarose beads by cyanogen bromide activation resulted in an immunoadsorbent with which a large amount of B. abortus-specific antibodies could be purified. The method described gave alkali-treated lipopolysaccharide binding efficiencies of up to 98%. There was little loss of alkali-treated lipopolysaccharide from the column after several pH shifts, allowing the immunoadsorbent to be regenerated and used repeatedly.
摘要
经碱处理的流产布鲁氏菌S1119.3脂多糖通过溴化氰活化偶联到琼脂糖珠上，得到一种免疫吸附剂，利用该免疫吸附剂可纯化大量流产布鲁氏菌特异性抗体。所述方法使碱处理的脂多糖结合效率高达98%。经过几次pH值变化后，柱中碱处理的脂多糖损失很少，使得该免疫吸附剂能够再生并重复使用。
相似文献
1
Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide.牛抗布鲁氏菌流产亚种脂多糖抗体的亲和纯化。
J Clin Microbiol. 1983 Feb;17(2):323-6. doi: 10.1128/jcm.17.2.323-326.1983.
2
Bovine monoclonal antibody specific for Brucella abortus lipopolysaccharide.针对流产布鲁氏菌脂多糖的牛单克隆抗体。
Vet Immunol Immunopathol. 1989 Jul;21(3-4):363-72. doi: 10.1016/0165-2427(89)90043-3.
3
Immunochemical studies on the binding properties of Brucella abortus lipopolysaccharides to bovine precipitating antibodies.布鲁氏菌流产菌株脂多糖与牛沉淀抗体结合特性的免疫化学研究
Mol Immunol. 1984 Nov;21(11):1123-9. doi: 10.1016/0161-5890(84)90123-8.
4
Haemolysis in gel test for detecting bovine antibodies to Brucella abortus lipopolysaccharide.用于检测牛抗流产布鲁氏菌脂多糖抗体的凝胶试验中的溶血现象。
Res Vet Sci. 1983 Jan;34(1):68-72.
5
Killing of Brucella abortus by bovine serum.牛血清对流产布鲁氏菌的杀灭作用
Infect Immun. 1988 Dec;56(12):3251-61. doi: 10.1128/iai.56.12.3251-3261.1988.
6
Localization of brucella antigens that elicit a humoral immune response in Brucella abortus-infected cells.在感染牛流产布鲁氏菌的细胞中引发体液免疫反应的布鲁氏菌抗原的定位。
Infect Immun. 1981 Dec;34(3):1000-7. doi: 10.1128/iai.34.3.1000-1007.1981.
7
Derivation of monoclonal antibodies against Brucella abortus antigens.
Vet Immunol Immunopathol. 1983 Jul;4(5-6):603-14. doi: 10.1016/0165-2427(83)90068-5.
8
Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 I immunoblot analysis of the antibody response to Brucella protein antigens in bovine brucellosis.流产布鲁氏菌与小肠结肠炎耶尔森氏菌0:9之间的血清学交叉反应：牛布鲁氏菌病中针对布鲁氏菌蛋白抗原的抗体反应的免疫印迹分析
Vet Microbiol. 1995 Dec;47(3-4):257-70. doi: 10.1016/0378-1135(95)00122-0.
9
Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection.布鲁氏菌感染中针对与光滑脂多糖复合物不同抗原的抗体反应。
Infect Immun. 1978 Sep;21(3):994-1002. doi: 10.1128/iai.21.3.994-1002.1978.
10
Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9.用流产布鲁氏菌S19疫苗接种或经小肠结肠炎耶尔森菌0:9血清型实验感染的牛，针对流产布鲁氏菌脂多糖和细胞质蛋白的体液免疫反应
Clin Diagn Lab Immunol. 1996 Jul;3(4):472-6. doi: 10.1128/cdli.3.4.472-476.1996.
引用本文的文献
1
Antibodies recognizing 20-hydroxyecdysone-dependent cell surface antigens during morphogenesis in Drosophila.在果蝇形态发生过程中识别20-羟基蜕皮激素依赖性细胞表面抗原的抗体。
Rouxs Arch Dev Biol. 1987 Oct;196(7):434-444. doi: 10.1007/BF00399143.
2
3
Cleavage of bovine immunoglobulin G1 in whey by an extracellular material from Brucella abortus.流产布鲁氏菌的一种细胞外物质对乳清中牛免疫球蛋白G1的裂解作用。
Can J Comp Med. 1985 Jul;49(3):337-9.
4
A "dipstick" enzyme immunoassay for detection of antibody to Brucella abortus in cattle sera.一种用于检测牛血清中布鲁氏菌流产亚种抗体的“试纸条”酶免疫测定法。
Can J Comp Med. 1985 Jul;49(3):298-302.
5
A comparison of five serological tests for bovine brucellosis.牛布鲁氏菌病五种血清学检测方法的比较。
Can J Vet Res. 1986 Oct;50(4):485-93.
6
Killing of Brucella abortus by bovine serum.牛血清对流产布鲁氏菌的杀灭作用
Infect Immun. 1988 Dec;56(12):3251-61. doi: 10.1128/iai.56.12.3251-3261.1988.
7
Bovine monoclonal antibody specific for Brucella abortus lipopolysaccharide.针对流产布鲁氏菌脂多糖的牛单克隆抗体。
Vet Immunol Immunopathol. 1989 Jul;21(3-4):363-72. doi: 10.1016/0165-2427(89)90043-3.
本文引用的文献
1
Ultracentrifugal studies of sera from cattle vaccinated or naturally infected with Brucella abortus.对接种过布鲁氏菌流产亚种疫苗或自然感染该病菌的牛的血清进行的超速离心研究。
Can J Comp Med Vet Sci. 1966 Oct;30(10):270-8.
2
Preparation of monospecific anti-Salmonella lipopolysaccharide antibodies by affinity chromatography.
Ann Immunol (Paris). 1981 Mar-Apr;132C(2):211-7. doi: 10.1016/0769-2625(81)90029-5.
3
Biological activities of Brucella abortus lipopolysaccharides.流产布鲁氏菌脂多糖的生物学活性
Infect Immun. 1981 Jan;31(1):362-70. doi: 10.1128/iai.31.1.362-370.1981.
4
Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.通过卤化氰将肽和蛋白质与多糖进行化学偶联。
Nature. 1967 Jun 24;214(5095):1302-4. doi: 10.1038/2141302a0.
5
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
J Biol Chem. 1970 Jun;245(12):3059-65.
6
Binding of covalent proteins to polysaccharides by cyanogen bromide and organic cyanates. I. Preparation of soluble glycine-, insulin- and ampicillin-dextran.溴化氰和有机氰酸盐介导的共价蛋白质与多糖的结合。I. 可溶性甘氨酸 - 葡聚糖、胰岛素 - 葡聚糖和氨苄青霉素 - 葡聚糖的制备。
Acta Chem Scand. 1971;25(5):1855-9. doi: 10.3891/acta.chem.scand.25-1855.
7
A method of trace iodination of proteins for immunologic studies.一种用于免疫学研究的蛋白质微量碘化方法。
Int Arch Allergy Appl Immunol. 1966;29(2):185-9. doi: 10.1159/000229699.
8
The serum and secretory immunoglobulins of cattle: characterization and quantitation.牛的血清和分泌型免疫球蛋白：特性与定量分析
J Immunol. 1972 Apr;108(4):965-76.
9
Isolation of rat transferrin using CNBr-activated sepharose 4B.
J Clin Chem Clin Biochem. 1976 Oct;14(10):475-8. doi: 10.1515/cclm.1976.14.1-12.475.
10
Improved method for the cyanogen bromide activation of agarose beads.
J Chromatogr. 1979 Apr 21;172:221-6. doi: 10.1016/s0021-9673(00)90957-9.
Zotero 插件