School of Marine Science and Technology, HuaiHai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.
Carbohydr Polym. 2013 Jan 30;92(1):629-32. doi: 10.1016/j.carbpol.2012.08.099. Epub 2012 Sep 1.
UDPG-pyrophosphorylase is the key enzyme involved in pullulan biosynthesis and pullulan production by Aureobasidium pullulans. In this study, effect of controlled pH on fermentation time, pullulan production, biomass, and UDPG-pyrophosphorylase activity was investigated. Pullulan yield increased to reach a maximum within 4 days, and maximum UDPG-pyrophosphorylase activity was observed at day 3, while the biomass continued to increase until the end of the experimental period. The A. pullulans isolated from sea mud grew well at relatively low pH. UDPG-pyrophosphorylase activity was affected by the controlled pH and reached a maximum at pH 5.5. Results indicated that UDPG-pyrophosphorylase activity was highly correlated with controlled pH and pullulan biosynthesis rate.
尿苷二磷酸葡萄糖焦磷酸化酶是参与普鲁兰生物合成的关键酶，也是出芽短梗霉生产普鲁兰的关键酶。在这项研究中，研究了控制 pH 值对发酵时间、普鲁兰产量、生物量和尿苷二磷酸葡萄糖焦磷酸化酶活性的影响。普鲁兰产量在 4 天内增加到最大值，最大尿苷二磷酸葡萄糖焦磷酸化酶活性出现在第 3 天，而生物量在实验结束前继续增加。从海泥中分离出的出芽短梗霉在较低的 pH 值下生长良好。尿苷二磷酸葡萄糖焦磷酸化酶活性受控制 pH 值的影响，在 pH 值为 5.5 时达到最大值。结果表明，尿苷二磷酸葡萄糖焦磷酸化酶活性与控制 pH 值和普鲁兰生物合成速率高度相关。
