Takahashi K, Ninomiya T
Department of Anatomy, Sapporo Medical College, Japan.
Neurosci Res. 1988 Dec;6(2):115-22. doi: 10.1016/0168-0102(88)90013-2.
Survival and myelination in dorsal root ganglion culture were observed using 6 kinds of modified commercial serum-free media: MEM, neMEM, alpha-MEM, DMEM, F12 medium and DF medium (see Materials and Methods). The results showed that the survival rates of dorsal root ganglia cultured in alpha-MEM and DF medium are the highest and that the rates of myelinated ganglia are the highest when alpha-MEM is used as culture medium, as compared with other serum-free media.
使用6种改良的市售无血清培养基：MEM、新MEM、α-MEM、DMEM、F12培养基和DF培养基（见材料与方法），观察背根神经节培养物中的存活和髓鞘形成情况。结果显示，与其他无血清培养基相比，在α-MEM和DF培养基中培养的背根神经节存活率最高，并且当使用α-MEM作为培养基时，有髓神经节的比例最高。
