Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
Methods Mol Biol. 2020;2123:393-400. doi: 10.1007/978-1-0716-0346-8_30.
Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.
马流感病毒在鸡胚或哺乳动物细胞中培养，通常是马肾传代细胞（MDCK），使用的方法与其他甲型流感病毒非常相似。与宿主适应性相关的突变在鸡蛋和 MDCK 细胞中都发生，但后者显示出更大的异质性，而鸡蛋通常是首选的宿主。马源 1 型 H7N7 和马源 2 型 H3N8 病毒在 11 日龄鸡胚中都能有效地复制，但我们发现马源 1 型病毒会杀死胚胎，而马源 2 型病毒则不会。
