Ilobi C P, Nicolson C, Taylor J, Mumford J A, Wood J M, Robertson J S
Division of Virology, National Institute for Biological Standards and Control, Potters Bar, U.K.
Arch Virol. 1998;143(5):891-901. doi: 10.1007/s007050050340.
Equine influenza viruses propagated in the laboratory in alternate hosts such as embryonated hens' eggs or mammalian cell culture have been analysed by HA sequencing and antigenically and their sequence compared to the original virus present in clinical material. In contrast to clinically derived human influenza virus which generally grows in MDCK cells without change, the data for equine influenza virus were less clear in that variants of equine virus were derived in both eggs and cells. The study indicated that the current use of eggs for equine influenza virus surveillance and vaccine production is entirely appropriate, but that care should be exercised when equine influenza vaccines are produced in eggs or on mammalian cell cultures.
在实验室中于替代宿主（如鸡胚或哺乳动物细胞培养物）中繁殖的马流感病毒，已通过血凝素（HA）测序、抗原分析进行了研究，并将其序列与临床材料中存在的原始病毒进行了比较。与临床分离的人类流感病毒通常在MDCK细胞中生长且无变化不同，马流感病毒的数据不太明确，因为在鸡蛋和细胞中均产生了马病毒变体。该研究表明，目前将鸡蛋用于马流感病毒监测和疫苗生产是完全合适的，但在鸡蛋或哺乳动物细胞培养物中生产马流感疫苗时应谨慎行事。
