Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, 221005, India.
Centre for Genetic Disorders, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
BMC Med Genet. 2020 Mar 14;21(1):53. doi: 10.1186/s12881-020-0993-6.
Adolescence is a distinctive stage of various changes and is noted as peak age for onset of many psychiatric disorders, especially linked to stress and depression. Several genetic variations are being increasingly known to be linked with stress and depression. The polymorphisms in two such genes, the BDNF and SLC1A3, have been reported to be linked with either depression/stress or with suicidal behaviour. These genes have not been validated in Indian population, and therefore there is a need to investigate these genes in Indian population. The present study was undertaken to test whether the known polymorphisms SLC1A3 C3590T, SLC1A3 C869G and BDNF G196A are associated or not with stress or depression in an eastern Indian population.
A case-control association study was performed with 108 cases having variable levels of stress and depression and 205 matched controls. Detection of stress and depression was done by using standard instruments as PSS and CES-D, respectively and demographic profile was obtained for each individual on the basis of personal data sheet. Genotyping for the selected polymorphisms was performed by PCR followed by restriction digestion.
The SNP SLC1A3 C3590T was found to be associated with stress and depression (p = 0.0042, OR = 2.072). Therefore, the T allele increases the risk by more than two folds for stress and depression in the present population. The other allele of SLC1A3, G869C, as well as BDNF G196A were not associated with stress or depression in the population studied.
SLC1A3 C3590T is a predisposition factor for stress and depression in an eastern Indian population, whereas SLC1A3 G869C and BDNF G196A were not found to be a risk factor. Therefore, presence of T allele of SLC1A3 C3590T, may predict the development of stress and depression in an individual. This may also help in the understanding of pathophysiology of the disease. However, these findings warrant a wider study in Indian populations and would be of significance in understanding the predisposition of stress and depression in this population.
青春期是各种变化的独特阶段，被认为是许多精神疾病发病的高峰期，尤其是与压力和抑郁有关的疾病。越来越多的遗传变异与压力和抑郁有关。两个这样的基因，BDNF 和 SLC1A3 的多态性，已经被报道与抑郁/压力或自杀行为有关。这些基因在印度人群中尚未得到验证，因此需要在印度人群中研究这些基因。本研究旨在检测已知的 SLC1A3 C3590T、SLC1A3 C869G 和 BDNF G196A 多态性是否与东部印度人群中的压力或抑郁有关。
采用病例对照关联研究，对 108 例具有不同程度压力和抑郁的病例和 205 例匹配对照进行研究。使用 PSS 和 CES-D 等标准工具检测压力和抑郁，根据个人数据表获取每个个体的人口统计学资料。通过 PCR 扩增 followed by restriction digestion 进行所选多态性的基因分型。
SNP SLC1A3 C3590T 与压力和抑郁有关（p=0.0042，OR=2.072）。因此，在本研究人群中，T 等位基因使压力和抑郁的风险增加了两倍以上。SLC1A3 的另一个等位基因 G869C 以及 BDNF G196A 与本研究人群中的压力或抑郁无关。
SLC1A3 C3590T 是东部印度人群中压力和抑郁的易感性因素，而 SLC1A3 G869C 和 BDNF G196A 不是风险因素。因此，SLC1A3 C3590T 的 T 等位基因的存在可能预测个体中压力和抑郁的发生。这也有助于理解疾病的病理生理学。然而，这些发现需要在印度人群中进行更广泛的研究，对于理解该人群中压力和抑郁的易感性具有重要意义。
