The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Yan'an Road, Hangzhou, 310006, China.
Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, China.
BMC Oral Health. 2020 Mar 14;20(1):72. doi: 10.1186/s12903-020-1042-8.
The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP).
Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software.
The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87, 18.75, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. S were 100%, and inter-groups similarity coefficients, i.e. S were less than 30%.
The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
本研究旨在调查口腔扁平苔藓（OLP）患者中白色念珠菌的流行情况和基因型谱。
分析了 OLP 患者和健康对照组中白色念珠菌菌株的阳性率和基因型谱。使用随机扩增多态性 DNA 和核糖体内部转录间隔区聚合酶链反应对这些菌株的 DNA 进行测序，然后使用 BLAST、UIV 带和 Vector NTI Suite 序列分析软件测量它们的遗传相似性。
侵蚀性 OLP、非侵蚀性 OLP 和正常个体中检测到的白色念珠菌菌株的患病率分别为 18.87%、18.75%和 7.92%。两种方法均揭示了四种不同的基因型。具体而言，仅在健康受试者中发现了 I 型；IIa 和 IIb 型在非侵蚀性 OLP 中发现，III 型在侵蚀性 OLP 中发现。组内相似系数，即 S 为 100%，组间相似系数，即 S 小于 30%。
OLP 中白色念珠菌的基因型结果表明白色念珠菌是内源性而非外源性感染。此外，白色念珠菌在 OLP 中的可能致病作用有助于更正确地认识 OLP 的发病机制、发展和进展，以及一些诊断和治疗策略。
