Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China; Departments of Medical Immunology & Medical Informatics, Medical College of Nantong University, Nantong 226001, China.
Departments of Medical Immunology & Medical Informatics, Medical College of Nantong University, Nantong 226001, China.
Hepatobiliary Pancreat Dis Int. 2020 Oct;19(5):449-454. doi: 10.1016/j.hbpd.2020.02.009. Epub 2020 Feb 29.
Hepatic Golgi protein-73 (GP73) expression is related to hepatocellular carcinoma (HCC) progression. The aim of this study was to investigate the dynamic expression of GP73 mRNA and protein during hepatocytes malignant transformation.
Human GP73 expressions in 88 HCC tissues and their self-control surrounding tissues were examined by immunohistochemistry, and survival time of HCC patients was evaluated by the Kaplan-Meier method. HCC model of Sprague-Dawley rats was made by diet containing 2-fluorenylacetamide. The rats were divided into the control, hepatocyte degeneration, precanceration, and HCC groups to observe GP73 protein and mRNA alterations during hepatocytes malignant transformation.
The GP73 expression was significantly higher in the cancerous tissues than that in the surrounding tissues, with shorter survival time, and the positive rates of GP73 protein in human HCC tissues were 53.3% at stage I, 84.0% at stage II, 84.6% at stage III, and 60.0% at stage IV, respectively. The positive rates of hepatic GP73 protein and mRNA in the rat models were none in the control group, 66.7% and 44.4% in the hepatocytes degeneration group, 88.9% and 77.8% in the hepatocytes precanceration group, and 100% in the HCC group, respectively. There was a positive correlation (r = 0.91, P<0.01) between hepatic GP73 and serum GP73 during rat hepatocytes malignant transformation.
Abnormal GP73 expression may be a sensitive and valuable biomarker in hepatocarcinogensis.
肝高尔基糖蛋白 73（GP73）的表达与肝细胞癌（HCC）的进展有关。本研究旨在探讨 GP73mRNA 和蛋白在肝细胞癌变过程中的动态表达。
采用免疫组化法检测 88 例 HCC 组织及其自身对照周围组织中 GP73 的表达，并采用 Kaplan-Meier 法评估 HCC 患者的生存时间。采用含 2-氟乙酰氨的饮食建立 Sprague-Dawley 大鼠 HCC 模型，将大鼠分为对照组、肝细胞变性组、癌前病变组和 HCC 组，观察肝细胞癌变过程中 GP73 蛋白和 mRNA 的变化。
癌组织中 GP73 的表达明显高于周围组织，生存时间较短，人 HCC 组织中 GP73 蛋白的阳性率分别为Ⅰ期 53.3%、Ⅱ期 84.0%、Ⅲ期 84.6%和Ⅳ期 60.0%。大鼠模型中肝 GP73 蛋白和 mRNA 的阳性率在对照组均为 0，肝细胞变性组分别为 66.7%和 44.4%，肝细胞癌前病变组分别为 88.9%和 77.8%，HCC 组均为 100%。大鼠肝细胞癌变过程中肝 GP73 与血清 GP73 呈正相关（r=0.91，P<0.01）。
异常的 GP73 表达可能是肝癌发生的一个敏感而有价值的标志物。
