Cvek Martin, Zahoranova Anna, Mrlik Miroslav, Sramkova Petra, Minarik Antonin, Sedlacik Michal
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Trida T. Bati 5678, 760 01 Zlín, Czech Republic.
Polymer Institute, Slovak Academy of Sciences, Dubravska cesta 9, 845 41 Bratislava, Slovakia.
Colloids Surf B Biointerfaces. 2020 Jun;190:110912. doi: 10.1016/j.colsurfb.2020.110912. Epub 2020 Mar 5.
Research on the subject of smart biomaterials has become a cornerstone of tissue engineering and regenerative medicine. Herein, the authors report on developing magnetic hydrogels that combine high biocompatibility and remarkable activity in magnetic fields. We fabricated magnetic hydrogels based on poly(2-ethyl-2-oxazoline) (POx) via living ring-opening cationic polymerization with in-situ embedding of the carbonyl iron (CI) particles. Investigation was made as to the effect exerted by the concentration of CI on magnetic, viscoelastic/magnetorheological properties, the degree of equilibrium swelling, and cytotoxicity. The hydrogels exhibited an open pore structure, as evidenced by computed tomography (CT) imaging. Susceptibility measurements revealed the concentration-dependent field-induced particle restructuration indicating elongation/contraction of the material, thereby determining the potential for magneto-mechanical stimulation of the cells. The POx-based magnetic hydrogels were amphiphilic in character, showing decrease in their capability to hold liquid alongside increase in CI concentration. Viscoelastic measurements suggested that interaction occurred between the particles and matrix based on inconsistency between the experimental storage modulus and the Krieger-Dougherty model. The synthesized materials exhibited excellent biocompatibility toward the 3T3 fibroblast cell line in tests of extract toxicity and direct contact cytotoxicity (ISO standards). The unique combination of properties exhibited by the material - magneto-mechanical activity and biocompatibility - could prove favorable in fields such as biomedicine and biomechanics.
智能生物材料的研究已成为组织工程和再生医学的基石。在此，作者报告了一种兼具高生物相容性和显著磁场活性的磁性水凝胶的研发情况。我们通过活性开环阳离子聚合反应，原位嵌入羰基铁（CI）颗粒，制备了基于聚（2-乙基-2-恶唑啉）（POx）的磁性水凝胶。研究了CI浓度对磁性、粘弹性/磁流变特性、平衡溶胀度和细胞毒性的影响。计算机断层扫描（CT）成像证明，水凝胶呈现开孔结构。磁化率测量结果显示，场致颗粒重构与CI浓度有关，表明材料会伸长/收缩，从而确定了对细胞进行磁机械刺激的可能性。基于POx的磁性水凝胶具有两亲性，随着CI浓度的增加，其持液能力下降。粘弹性测量结果表明，由于实验储能模量与Krieger-Dougherty模型不一致，颗粒与基质之间发生了相互作用。在提取物毒性和直接接触细胞毒性测试（ISO标准）中，合成材料对3T3成纤维细胞系表现出优异的生物相容性。该材料所展现的独特性能组合——磁机械活性和生物相容性——在生物医学和生物力学等领域可能具有优势。
