Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, Heilongjiang, China.
Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences, Key Laboratory of Soybean Cultivation of Ministry of Agriculture PR China, Harbin Heilongjiang, China.
Funct Plant Biol. 2019 Mar;46(4):304-313. doi: 10.1071/FP18111.
Phytophthora root and stem rot of soybean (Glycine max (L.) Merr.) caused by Phytophthora sojae is a destructive disease worldwide. The enzyme 4-coumarate: CoA ligase (4CL) has been extensively studied with regard to plant responses to pathogens. However, the molecular mechanism of the response of soybean 4CL to P. sojae remains unclear. In a previous study, a highly upregulated 4CL homologue was characterised through suppressive subtractive hybridisation library and cDNA microarrays, in the resistant soybean cultivar 'Suinong 10' after infection with P. sojae race 1. Here, we isolated the full-length EST, and designated as GmPI4L (P. sojae-inducible 4CL gene) in this study, which is a novel member of the soybean 4CL gene family. GmPI4L has 34-43% over all amino acid sequence identity with other plant 4CLs. Overexpression of GmPI4L enhances resistance to P. sojae in transgenic soybean plants. The GmPI4L is located in the cell membrane when transiently expressed in Arabidopsis protoplasts. Further analyses showed that the contents of daidzein, genistein, and the relative content of glyceollins are significantly increased in overexpression GmPI4L soybeans. Taken together, these results suggested that GmPI4L plays an important role in response to P. sojae infection, possibly by enhancing the content of glyceollins, daidzein, and genistein in soybean.
大豆疫霉根腐和茎腐病（Phytophthora root and stem rot of soybean，Glycine max (L.) Merr.）由大豆疫霉（Phytophthora sojae）引起，是一种全球性的破坏性疾病。植物对病原体的反应方面，4-香豆酸辅酶 A 连接酶（4-coumarate: CoA ligase，4CL）已被广泛研究。然而，大豆 4CL 对 P. sojae 反应的分子机制尚不清楚。在之前的研究中，通过抑制差减杂交文库和 cDNA 微阵列，在抗大豆品种 '苏农 10' 感染 P. sojae 1 号小种后，鉴定出一个高度上调的 4CL 同源物。在本研究中，我们分离了全长 EST，并将其命名为 GmPI4L（P. sojae 诱导的 4CL 基因），它是大豆 4CL 基因家族的一个新成员。GmPI4L 与其他植物 4CL 的所有氨基酸序列同一性为 34-43%。过表达 GmPI4L 增强了转基因大豆植株对 P. sojae 的抗性。GmPI4L 在拟南芥原生质体中瞬时表达时位于细胞膜上。进一步的分析表明，过表达 GmPI4L 的大豆中大豆苷元、染料木黄酮和大豆异黄酮相对含量的含量显著增加。综上所述，这些结果表明 GmPI4L 在响应 P. sojae 感染中发挥重要作用，可能通过增强大豆中大豆异黄酮、大豆苷元和染料木黄酮的含量来实现。
